By screening of HLCVECs cDNA expression library using sera from immunized mice with HLCVECs, the functional genes associated with tumor endothelium or angiogenesis were identified. The modified SEREX, xenogeneic functional serum screening, was demonstrated to be effective for isolation and identification of antigen genes of tumor endothelium, and also for other tumor cell antigen genes. These antigen genes obtained in this study could be a valuable resource for basic and clinical studies of tumor angiogenesis, thus facilitating the development of anti- angiogenesis targeting therapy of tumors.
Lithium extraction from seawater and brine is an important approach to solving the short supply of lithium resource. Development of ion-sieve adsorption materials is the key of realizing industrial application of lithium extraction from seawater and brine with high ratio of magnesium to lithium. Although the synthetic process is complicated, with plenty of influencing factors, levels, and large amounts of data, this research successfully optimized the synthesis conditions by orthogonal experiment and statistical analysis. The achievements will lay solid foundation for the development of lithium adsorption materials.
The modified diatomite was prepared by calcination and alkali washing, and the feasibility of using modified diatomite for enhanced coagulation treatment of slightly polluted seawater was investigated in this study. SEM and BET analysis showed that the raw diatomite contained impurities, which were removed after refined and purified treatment. The use of diatomite as a coagulant aid improved interparticle bridging thus incorporating the cells into flocs more efficiently, producing settleable flocs of greater density, size and strength. The enhanced coagulation with ferric sulfate and modified diatomite improved the removal efficiency of turbidity, ammonia nitrogen, total phosphorus and CODMn in seawater samples. At the optimal diatomite dosage of 60 mg/L and ferric sulfate dosage of 6 mg/L, the removal efficiency of turbidity, NH3-N, TP and CODMn reached 93.8%, 26.3%, 94.8% and 44.4% respectively. It has been proved that the enhanced coagulation through the ferric sulfate and modified diatomite, as coagulant aid and adsorbent, is more efficient and environmentally than using traditional metal coagulant only for treatment of slightly polluted seawater.
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