Mitochondrial genome sequences were first determined and analyzed for a Korean endemic freshwater mussel Nodularia breviconcha (synonym Nodularia douglasiae sinuolatus; Unionidae, Unionida, Bivalvia). The complete mitochondrial genome was 15,741 bp in length, including 13 protein-coding genes (PCGs), 22 tRNA genes, and 2 rRNA genes. The overall GC content of mitochondrial genome for N. breviconcha was 34.3%. Phylogenetic analysis of 18 species within the family Unionidae suggested that Nodularia douglasiae is the most closely related to N. breviconcha. Our study will provide baseline, but important information for future research on ecological and genetic/genomic characteristics of this species.
The Antarctic toothfish, Dissostichus mawsoni, serves as a valuable fishery resource around the Antarctic Continent since 1997, managed by the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR). Although delineating genetic or stock structure of populations is crucial for improving fishery management of this species, its number of genetic populations and genetic diversity levels remain ambiguous. In the present study, we assessed the population genetic and phylogeographic structure of the Antarctic toothfish across 20 geographic localities spanning from Subareas 88 (88.1, 88.2, and 88.3) to Subareas 58 (58.4 and 58.5) by using mitochondrial DNA (mtDNA) cytochrome oxidase I (COI) and 16S rRNA (16S) sequences and seven nuclear microsatellite loci. MtDNA revealed a low level of polymorphism (h = 0.571, π = 0.0006) with 40 haplotypes in 392 individuals, connected only by 1–5 mutational steps, which is indicative of shallow evolutionary history. Microsatellites showed a range of allelic richness (AR) from 6.328 (88.3 RB3) to 7.274 (88.3 RB6) within populations. Overall genetic diversity was generally higher in Subareas 58 than in Subareas 88, suggesting that effective population size (NE) is larger in Subareas 58. The results of population analyses using microsatellites suggest that the sampled populations are likely to comprise a well-admixed single gene pool (i.e., one genetic stock), perhaps due to high contemporary gene flow occurring during the prolonged larval phase of this fish. However, given weak, but significant microsatellite differentiation found in six population-pairs, the possibility of existence of multiple genetic populations could not be completely excluded. The mtDNA AMOVA suggests a genetic break between the Subareas 88 and 58 groups (FCT = 0.011, P = 0.004). Moreover, mtDNA genetic distances (FST) between populations were proportionally greater as geographic distances increase. The patterns of isolation by distance (IBD) shown only in mtDNA, but not in microsatellites might suggest that population differentiation or divergence processes underwent faster in mtDNA than microsatellites, due to its NE being only one-quarter of nuclear DNA. Temporal stability in the genetic structure of D. mawsoni is also indicated by the results of no genetic differentiation between juveniles and adults. The findings of this study will help to design effective stock management strategies for this valuable fishery resource. We suggest that a long-term genetic monitoring is needed to understand the population structure and dynamics of toothfish in response to ongoing climate changes.
Population or habitat connectivity is a key component in maintaining species and community-level regional biodiversity as well as intraspecific genetic diversity. Ongoing human activities cause habitat destruction and fragmentation, which exacerbate the connectivity due to restricted animal movements across local habitats, eventually resulting in the loss of biodiversity. The Baekdudaegan Mountain Range (BMR) on the Korean Peninsula represents “biodiversity hotspots” and eight of the 22 Korean national parks are located within the BMR. Given the striped field mouse (Apodemus agrarius) is the most common and ecologically important small mammals in these protected areas, the population genetic assessment of this species will allow for identifying “genetic diversity hotspots” and also “genetic barriers” that may hinder gene flow, and will therefore inform on effective conservation and management efforts for the national park habitats. We collected samples from hair, tail, or buccal swabs for 252 A. agrarius individuals in 2015 and 2019. By using mitochondrial DNA cytochrome b (cyt b) sequences and nine microsatellite loci, we determined levels of genetic diversity, genetic differentiation, and gene flow among eight national park populations of A. agrarius along the BMR. We found high levels of genetic diversity but the occurrences of inbreeding for all the nine samples analyzed. Our results also indicated that there was detectable temporal genetic variation between the 2015 and 2019 populations in the Jirisan National Park, which is probably due to a short-term decline in genetic diversity caused by reduced population sizes. We also found a well-admixed shared gene pool among the national park populations. However, a significant positive correlation between geographic and genetic distances was detected only in mtDNA but not microsatellites, which might be attributed to different dispersal patterns between sexes. There was a genetic barrier to animal movements around the Woraksan National Park areas. The poor habitat connectivity surrounding these areas can be improved by establishing an ecological corridor. Our findings of the presence of genetic barriers in some protected areas provide insights into the conservation and management efforts to improve the population or habitat connectivity among the national parks.
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