The instruments utilizing fluorescent dyes in cutaneous disorders include Wood's ramp for vitiligo and indocyanine green fluorescence for sentinel lymph node detection. 1,2 MolecuLight i:X® (MolecuLight Inc.) makes it possible to visualize the adhesion of bacteria over 10,000 CFU/g by recognizing porphyrin produced by staphylococci, etc., as red fluorescence and pyoverdine produced by Pseudomonas aeruginosa as cyan fluorescence, with a peak excitation light of 405 nm 3 . Cyan fluorescence has a wavelength of 501-542.5 nm (±1.5 nm), while red fluorescence has a wavelength of 601-664 nm (±1.5 nm). 3 It is believed that using fluorescence image observation can estimate the adhesion of bacteria through non-invasive and real-time observation, while appropriately setting the range of debridement improved the healing rate of ulcers. 4,5 In this study, we investigated clinical utility and accuracy of MolecuLight i:X® to evaluate bacterial clustering on intractable ulcers in Asian patients.
| C A S E REP ORTFrom a total of 20 cases, 55 photographs were taken from May to December 2021 in this study. Table 1 summarizes the enrolled patients and ulcer profile. Aggravation/improvement of ulcers and changes in fluorescence over time were collated. Furthermore, the usefulness of MolecuLight i:X® as a non-invasive imaging tool was analyzed by collating the results of wound cultures and fluorescence, then calculating the sensitivity and specificity of fluorescence against the wound cultures.
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