The deployment of Li metal batteries has been significantly tethered by uncontrollable lithium dendrite growth, especially in heavy-duty operations. Herein, we implement an in situ surface transformation tactic exploiting the vapor-phase solid–gas reaction to construct an artificial solid-electrolyte interphase (SEI) of Li2Se on Li metal anodes. The conformal Li2Se layer with high ionic diffusivity but poor electron conductivity effectively restrains the Li/Li+ redox conversion to the Li/Li2Se interface, and further renders a smooth and chunky Li deposition through homogenized Li+ flux and promoted redox kinetics. Consequently, the as-fabricated Li@Li2Se electrodes demonstrate superb cycling stability in symmetric cells at both high capacity and current density. The merits of inhibited dendrite growth and side reactions on the stabilized Li@Li2Se anode are further manifested in Li–O2 batteries, greatly extending the cycling stability and energy efficiency.
A Master Sintering Curve (MSC) for rutile TiO2 was constructed for Pressure-less sintering using constant heating rate dilatometry data based on the combined-stage sintering model. Construction of the master sintering curve is described and the validation is proved with rutile TiO2 under different thermal histories. The concept of master sintering can be used to predict the sintering shrinkage and final density and calculate the activation energy, and a value of 105 KJ/mol for TiO2 was obtained. With one temperature dependent parameter determined experimentally, it became possible to describe accurately the densification behavior of TiO2 from the initial to final stages of sintering.
Background Epstein-Barr virus (EBV) has a biphasic infection cycle consisting of a latent and a lytic replicative phase. The product of immediate-early gene BRLF1, Rta, is able to disrupt the latency phase in epithelial cells and certain B-cell lines. The protein Rta is a frequent target of the EBV-induced cytotoxic T cell response. In spite of our good understanding of this protein, little is known for the gene polymorphism of BRLF1. Results BRLF1 gene was successfully amplified in 34 EBV-associated gastric carcinomas (EBVaGCs), 57 nasopharyngeal carcinomas (NPCs) and 28 throat washings (TWs) samples from healthy donors followed by PCR-direct sequencing. Fourteen loci were found to be affected by amino acid changes, 17 loci by silent nucleotide changes. According to the phylogenetic tree, 5 distinct subtypes of BRLF1 were identified, and 2 subtypes BR1-A and BR1-C were detected in 42.9% (51/119), 42.0% (50/119) of samples, respectively. The distribution of these 2 subtypes among 3 types of specimens was significantly different. The subtype BR1-A preferentially existed in healthy donors, while BR1-C was seen more in biopsies of NPC. A silent mutation A/G was detected in all the isolates. Among 3 functional domains, the dimerization domain of Rta showed a stably conserved sequence, while DNA binding and transactivation domains were detected to have multiple mutations. Three of 16 CTL epitopes, NAA, QKE and ERP, were affected by amino acid changes. Epitope ERP was relatively conserved; epitopes NAA and QKE harbored more mutations. Conclusions This first detailed investigation of sequence variations in BRLF1 gene has identified 5 distinct subtypes. Two subtypes BR1-A and BR1-C are the dominant genotypes of BRLF1. The subtype BR1-C is more frequent in NPCs, while BR1-A preferentially presents in healthy donors. BR1-C may be associated with the tumorigenesis of NPC.
The Epstein-Barr virus (EBV) BamHI-C fragment rightward reading frame 1 (BCRF1)-coded viral interleukin-10 (vIL-10), exhibits high homology with human interleukin-10 (hIL-10) gene. The protein product vIL-10, which shares some functional properties with hIL-10, primarily mediates immunosuppressive functions. To characterize the variations of the vIL-10 gene and to explore the association between vIL-10 gene variations and EBV associated diseases, the vIL-10 gene was analyzed (using direct sequencing) in 41 cases of EBV-associated gastric carcinoma (EBVaGC), 83 nasopharyngeal carcinoma biopsies, and 40 throat washing samples from healthy donors in Northern China. One silent mutation (c9980a) was observed in the majority of EBVaGC, nasopharyngeal carcinoma and throat washing samples (134/164, 81.7%). Two consensus mutations (V6M and G23S) were identified in the signal peptide region in some nasopharyngeal carcinoma and throat washing isolates. These results indicate that the pattern B95-8 (identical sequence to B95-8) is the dominant type among the EBV isolates from Northern China, while the pattern SPM (mutation in the signal peptide present only in nasopharyngeal carcinoma and throat washing isolates) seems more relevant with the EBV-positive nasopharyngeal and laryngopharyngeal epithelial cells. The conservation of vIL-10, with a few variations, suggests the critical role of the vIL-10 gene for EBV in gaining an advantage over the host's immune system.
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