The technique for replicating hepatitis C virus (HCV) in cell culture has been modified and the susceptibility of the cells of various origin to HCV upon their infection with HCV-containing sera has been compared. The viral load on the fifth day post-infection has been assessed by reverse transcriptase polymerase chain reaction technique. The highest infection and replication efficacy have been found in cells of rat Gasser’s ganglion neurinoma. The peculiar features of the mitotic index and the anomalous forms of the mitosis have been studied in HCV-infected cells. The data presented may be used as a basis for the experimental model of HCV infection in vitro suitable for studying the effects of antiviral drugs on the infection caused by the cytopathogenic variant of HCV
This paper presents some results concerning investigation of antiviral activity of a new plant origin preparation-neoflazidum, a form of proteflazidum-using a model of hepatitis C virus-producing Jurkat cell culture (Jurkat HCV) which has been previously transfected with complementary DNA preparations isolated from different types of hepatitis C virus (HCV). To obtain these preparations, HCV RNA preparations were isolated from viral material of HCV-diseased patients, the transfecting agent Turbofect being used. The cytotoxic dose (CD 50) was shown to be 37.2 μg/ml, the effective dose (ED 50) being 0.46 μg/ml for the HCV type 3a; this dose, however, was as high as 3.7 μg/ml when neoflazidum had been tested for HCV type 1b. Therefore, the selectivity index for the HCV type 3a reached 80.8 in the Jurkat HCV-3a cell system, being as low as 10.0 for the HCV type 1b. Using a model system RNAP T7, the antiviral neoflazidum activity was demonstrated to be realized due to interferons α-and γ-induction and RNA synthesis inhibition.
The study of the effect of endogenous cannabimimetic compound -N-stearoylethanolamine (NSE) on the hepatitis C virus (HCV) reproduction. Methods. The model of the surrogate HCV is a bovine diarrhea virus; cell culture model is cells transfected with cDNA of the human HCV and molecular docking has been used. Results. In vitro studies showed that NSE effectively inhibited the reproduction of a surrogate HCV in both MDBK cells and transfected Jurkat cells. Molecular docking suggested that NSE can bind to the active centers of both NS3 serine protease and HCV NS5B-polymerase and has an inhibitory effect on their activity. Conclusions. The obtained data confirm that using NSE is promising for the development of antiviral drug to suppress the HCV activity.
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