A resolution-enhanced integral imaging microscope that uses lens array shifting is proposed in this study. The lens shift method maintains the same field of view of the reconstructed orthographic view images with increased spatial density. In this study, multiple sets of the elemental images were captured with horizontal and vertical shifts of the micro lens array and combined together to form a single set of the elemental images. From the combined elemental images, orthographic view images and depth slice images of the microscopic specimen were generated with enhanced resolution.
In an integral imaging display, the computer-generated integral imaging method has been widely used to create the elemental images from a given three-dimensional object data. Long processing time, however, has been problematic especially when the three-dimensional object data set or the number of the elemental lenses are large. In this paper, we propose an image space parallel processing method, which is implemented by using Open Computer Language (OpenCL) for rapid generation of the elemental images sets from large three-dimensional volume data. Using the proposed technique, it is possible to realize a real-time interactive integral imaging display system for 3D volume data constructed from computational tomography (CT) or magnetic resonance imaging (MRI) data.
A real-time interactive orthographic-view image display of integral imaging (II) microscopy that includes the generation of intermediate-view elemental images (IVEIs) for resolution enhancement is proposed. Unlike the conventional II microscopes, parallel processing through a graphics processing unit is required for real-time display that generates the IVEIs and interactive orthographic-view images in high speed, according to the user interactive input. The real-time directional-view display for the specimen for which 3D information is acquired through II microscopy is successfully demonstrated by using resolution-enhanced elemental image arrays. A user interactive feature is also satisfied in the proposed real-time interactive display for II microscopy.
An integral imaging microscopy (IIM) system with improved depth-of-field (DoF) using a custom-designed bifocal polarization-dependent liquid-crystalline polymer micro lens array (LCP-MLA) is proposed. The implemented MLA has improved electro-optical properties such as a small focal ratio, high fill factor, low driving voltage, and fast switching speed, utilizing a well-aligned reactive mesogen on the imprinted reverse shape of the lens and a polarization switching layer. A bifocal MLA switches its focal length according to the polarization angle and acquires different DoF information of the specimen. After two elemental image arrays are captured, the depth-slices are reconstructed and combined to provide a widened DoF. The fabricated bifocal MLA consists of two identical polarization-dependent LCP-MLAs with 1.6 mm and f/16 focal ratio. Our experimental results confirmed that the proposed system improves the DoF of IIM without the need for mechanical manipulation.
A depth-of-field enhancement method for integral imaging microscopy system using a spatial multiplexing structure consisting of a beamsplitter with dual video channels and micro lens arrays is proposed. A computational integral imaging reconstruction algorithm generates two sets of depth-sliced images for the acquired depth information of the captured elemental image arrays and the well-focused depth-slices of both image sets are combined where each is focused on a different depth plane of the specimen. A prototype is implemented, and the experimental results demonstrate that the depth-of-field of the reconstructed images in the proposed integral imaging microscopy is significantly increased compared with conventional integral imaging microscopy systems.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.