The biological response of fetal rat calvarial cells on a TiO2 nanotubular surface (Ti-NT) was evaluated by cell viability assay, alkaline phosphatase (ALP) activity and reverse transcription polymerase chain reaction (RT-PCR) analysis. The cell viability assay showed no significant difference between the Ti-NT and smooth titanium surfaces (Ti-S). Ti-NT had better cellular responses with regard to the ALP activity and bone-associated markers, such as bone sialoprotein and osteocalcin mRNA than Ti-S. These results suggest that Ti-NT stimulate the differentiation into osteoblasts of fetal rat calvarial cells, potentially contributing to rapid osseointegration.
This study examined the cell response to a TiO2 nanotubular surface (Ti-NT) for future biomedical applications. The level of cell attachment and spreading at 20 min and 60 min was evaluated by SEM. IL-6 and PGE2 secretion was evaluated by ELISA. In SEM analysis, the Ti-NT surface had more fully spread cells compared to the machined titanium surface (Ti-S). ELISA revealed that the level of IL-6 and PGE2 production was higher on the Ti-NT than on the Ti-S. These results suggest that a surface treatment with a nanotubular TiO2 surface enhances the early osteoblast responses, such as cell spreading and cytokine release, which are important for subsequent cell functions and bone healing in vivo.
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