The 95% aqueous EtOH extract was obtanied from the fruiting body of Russula subnigricans. Repeated silica gel column chromatography and preparative TLC afforded one fatty acid and three chlorinated hydroquinone derivatives. They were identified as nonadecanoic acid (1), 2,6-dichloro-4-methoxyphenol (2), russuphelin A (3), and russuphelin E (4) on the basis of several spectral data (MS, 1 H and 13 C-NMR, including HMBC).
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