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Aims: The objective of the study was to optimize the submerged culture conditions for the production of exopolysaccharide from Paecilomyces sinclairii. Methods and Results: The optimal temperature and initial pH for exopolysaccharide production by Paecilomyces sinclairii in shake flask culture were found to be 30°C and 6AE0, respectively. Sucrose (60 g l )1 ) and corn steep powder (10 g l )1 ) were the most suitable carbon and nitrogen source for exopolysaccharide production. Conclusions: Under optimal culture medium, the maximum exopolysaccharide concentration in a 5-l stirred-tank fermenter indicated 7AE4 g l )1 , which was approximately three times higher than that in basal medium. The maximum specific growth rates (l max ) and yield coefficient (Y P/S ) in the optimal culture medium was 0AE16 h )1 and 0AE19, respectively. Significance and Impact of the Study: The optimal culture conditions reported in this article can be widely applied to the processes for submerged cultures of other mushrooms.

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