In this study, 3D polycaprolactone (PCL) scaffolds were fabricated by 3D printing technique. The macro/nano morphology of, 3D PCL scaffolds surface was etched with oxygen plasma. Acrylic acid (AA) plasma-polymerization was performed to functionalize the macro/nano surface with carboxyl groups and then collagen was immobilized with plasma-polymerized 3D PCL scaffolds. After O2 plasma and AA plasma-polymerization, contact angles were decreased. The FE-SEM and AFM results showed that O2 plasma is increased the surface roughness. The MTT assay results showed that proliferation of the M3CT3-E1 cells increased on the oxygen plasma treated and collagen immobilized 3D PCL scaffolds.
Staphylococcus aureus is a representative of gram-positive bacteria that causes skin infection, respiratory diseases, and burned tissue infections. The aim of this study was to evaluate the sterilizing efficiency of an atmospheric-pressure plasma jet (APPJ) on S. aureus adhered on a titanium surface. During the APPJ sterilization, the plasma gases used were Ar, Ar+N2, and Ar+O2. With increasing APPJ treatment time, the viability of S. aureus decreased. The addition of O2 gas to Ar gas resulted in a higher sterilizing efficiency than the addition of other groups. Plasma exposure induced bacterial oxidative stress, and it was confirmed that the cell membrane was seriously damaged by the production of reactive oxygen species. Our finding suggests that the APPJ is an effective tool for clinical antimicrobial therapy.
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