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Increasing energy expenditure by activating thermogenesis in brown and beige adipocytes is a critical approach to protect against obesity. Here, we investigated the action and mechanism of a natural polymethoxyflavone on adaptive thermogenesis in high-fat diet-induced obesity mouse model. Nobiletin treatment significantly ameliorated obesity, alleviated the whitening of brown adipose tissue, and promoted browning of white adipose tissue in mice fed a high-fat diet. Gut microbiota analysis and metabolomic profiling revealed that nobiletin treatment resulted in a composition shift in the gut microbiota thereby altering fermentation products acetate levels in the host feces and serum. Further, transplantation of the microbiota from nobiletin-treated mice to microbiota-depleted mice activated brown adipose tissue activity, promoted beige adipocytes formation, and improved high-fat diet-induced obesity. Our results indicate that nobiletin could be used as a dietary therapy to prevent HFD-induced obesity, and provide a potential target-specific gut microbial species-driven mechanism for activating thermogenesis in brown and beige adipocytes.

Background: Preeclampsia is one form of hypertensive disorders, responsible for severe morbidity, long-term disability and maternal and newborn mortality worldwide. Studies have assessed the role of diet on the risk of preeclampsia using various dietary patterns, however, the association between dietary pattern and the risk of preeclampsia is not entirely clear. This study aimed to determine the association between maternal dietary patterns and the risk of preeclampsia through a meta-analysis of observational studies. Methods: Electronic literature was searched in 3 databases, including PubMed, Cochrane Library, and Web of Science, for articles published up to November 2020 that examined the association between dietary patterns and preeclampsia. Only studies considered observational were included. Two authors independently performed study selection and data extraction. Pooled effect sizes of eligible studies were estimated by using randomeffects models for healthy diet patterns and fixed-effects models for western diet patterns. Results: A total of 12 articles reporting 25 studies were selected for this study. Results from 11 articles reporting 22 studies were pooled. Healthy dietary patterns (16 studies) and western dietary pattern (6 studies) were identified. Higher adherence to a healthy dietary pattern was significantly associated with a reduced risk of preeclampsia (OR (95% CI): 0.79 (0.73e0.85), I 2 ¼ 57.2%, P ¼ 0.009),

Gut microbiota bears adaptive potential to different environments, but little is known regarding its responses to acute high-altitude exposure. This study aimed to evaluate the microbial changes after acute exposure to simulated high-altitude hypoxia. C57BL/6J mice were divided into hypoxia and normoxia groups. The hypoxia group was exposed to a simulated altitude of 5500 m for 24 hours above sea level. The normoxia group was maintained in low-altitude of 10 m above sea level. Colonic microbiota was analyzed using 16S rRNA V4 gene sequencing. Compared with the normoxia group, shannon, simpson and Akkermansia were signi cantly increased, while Firmicutes to Bacteroidetes ratio and Bi dobacterium were signi cantly decreased in the hypoxia group. The hypoxia group exhibited lower mobile element containing and higher potentially pathogenic and stress tolerant phenotypes than those in the normoxia group. Functional analysis indicated that environmental information processing was signi cantly lower, metabolism, cellular processes and organismal systems were signi cantly higher in the hypoxia group than those in the normoxia group. In conclusion, acute exposure to simulated highaltitude hypoxia alters gut microbiota diversity and composition, which may provide a potential target to alleviate acute high-altitude diseases.