We used multilocus sequence typing (MLST) to analyze the diversity of natural isolates of , the most important microorganism in alcoholic fermentation. Six loci, ADP1, RPN2, GLN4, ACC1, MET4, and NUP116, in genome were selected as MLST markers. To investigate genetic diversity within , 42 isolated from natural sources in Korea as well as six obtained from Genbank and four industrial were examined using MLST. Twenty-six polymorphic sites were found in the six loci. Among them, ACC1 had the most genetic variation with eight polymorphic sites. MLST differentiated the 52 strains into three clades. Alcohol fermentation results revealed that in Clade III produced less alcohol than those in Clades I and II. These results suggested that MLST is a powerful tool to differentiate and can potentially be used to select suitable for industrial use.
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