This study was undertaken to quantify the rate of nitrogen excretion by the cultured Japanese flounder as a function of body weight and water temperature. Measurements of the d i d rate of nitrogen excretion for the cultured flounder were made at mean water temperature of about 16,19, and 23 C. The excretion rate of nilmgen was estimated by adding the nitrogen excreted in the forms of ammonia and urea nitrogens. The diurnal pattern in the excretion rate of nitrogen was governed mainly by the feeding time. The proportions of excreted ammonia to total nitrogen were 77.9% at 16 C, 84.5% at 19 C, and 83.2% at 23 C. The average was 81.9%. The relationship between the body weight and excretion rate of nitrogen (EN, mg N / h h per h) is expressed as EN = a,.W', where a, = nitrogen excretion rate at temperature 8. The parameter a, is dependent on water temperature, such that a, = Q~. Q~, ( -~) , where am = nitrogen excretion rate at 20 C, and Qo, = temperature coefficient. The values of the kinetics parameters for the cultured flounder were found to be 7 = 0.57, am = 0.060, and Q, = 1.12.The water supply for a culture pond of the Japanese flounder Paralichthys oliva-
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