Thioredoxin-binding protein-2 (TBP-2)/vitamin D 3 upregulated protein 1 is an endogenous molecule interacting with thioredoxin (TRX), negatively regulating TRX function, and being implicated in the suppression of tumor development and metastasis. We found that TBP-2 ectopically expressed in the breast cancer cell line MCF-7 was localized predominantly in the nucleus exhibiting growth suppressive activity. The nuclear accumulation of endogenous TBP-2 protein was also demonstrated when the cells were treated with an anticancer drug, suberoylanilide hydroxamic acid. To investigate the mechanism underlying the nuclear localization, we performed a yeast two-hybrid screening and identified importin ␣ 1 (Rch1) as a protein interacting with TBP-2. The physical interaction between TBP-2 and Rch1 was confirmed with a glutathione S-transferase pull-down assay. The interaction of TBP-2 was specific to Rch1 among other importin ␣ subfamilies (Qip1 and NPI-1), and amino acids 1-227 of TBP-2 were sufficient for both the interaction with Rch1 and the nuclear localization, although there is no typical nuclear localization signal in this sequence. The expression of short interfering RNA of Rch1 suppressed suberoylanilide hydroxamic acid-induced nuclear accumulation of TBP-2. Collectively, our results strongly suggest that an interaction with importin system is required for TBP-2 nuclear translocation and growth control tightly associated with TRX-dependent redox regulation of transcription factors.
Thioredoxin (TRX) has a role in a variety of biological processes, including cytoprotection and the activation of transcription factors. Nerve growth factor (NGF) is a major survival factor of sympathetic neurons and promotes neurite outgrowth in rat pheochromocytoma PC12 cells. In this study, we showed that NGF induces TRX expression at protein and mRNA levels. NGF activated the TRX gene through a regulatory region positioned from -263 to -217 bp, containing the cAMP-responsive element (CRE). Insertion of a mutation in the CRE in this region abolished the response to NGF. NGF induced binding of CRE-binding protein to the CRE of the TRX promoter in an electrophoretic mobility shift assay. NGF also induced nuclear translocation of TRX. 2'-Amino-3'-methoxyflavone, an inhibitor of mitogen-activated protein kinase kinase, which is a known inhibitor of NGF-dependent differentiation in PC12 cells, suppressed the NGF-dependent expression and nuclear translocation of TRX. Overexpression of mutant TRX (32S/35S) or TRX antisense vector blocked the neurite outgrowth of PC12 cells by NGF. Overexpression of mutant TRX (C32S/C35S) suppressed the NGF-dependent activation of the CRE-mediated c-fos reporter gene. These results suggest that TRX plays a critical regulatory role in NGF-mediated signal transduction and outgrowth in PC12 cells.
The dynamical structures of molecules of normal alkanes (C5–C14) and normal fatty acids (C8 and C9) in the liquid state were estimated through analyses of their self-diffusion coefficients (D), viscosities (η), molar volumes, and 13CNMR spin-lattice relaxation time (T1). The apparent hydrodynamic radius evaluated from D and η for the alkanes was ca. 2.5×10−10 m and that for the fatty acids, ca. 3.1×10−10 m, irrespective of their hydrocarbon chain length: The former value is almost equal to that of the van der Waals radius (2.4×10−10 m) of the cross section of a hydrocarbon chain; the latter is in agreement with that of the normal alcohols reported in a previous paper. It is thus concluded that in the pure liquid of a rod-like compound such as normal alkane, alcohol, and fatty acid, the free rotational (end-over-end) as well as the transverse motion of the rod-like molecule is severely restricted due to the entanglements of the molecules; only a longitudinal translation is allowed. In addition, the infrared-spectrum results suggest that a unit in translational motion for a normal fatty acid is a dimer.
Vascular endothelial growth factor (VEGF) is a central regulator of blood vessel morphogenesis, although its role in patterning of endothelial cells into vascular networks is not fully understood. It has been suggested that binding of soluble VEGF to extracellular matrix components causes spatially restricted cues that guide endothelial cells into network patterns. Yet, current evidence for such a mechanism remains indirect. In this study, we quantitatively analyse the dynamics of VEGF retention in a controlled in vitro situation of human umbilical vascular endothelial cells (HUVECs) in Matrigel. We show that fluorescent VEGF accumulates in pericellular areas and colocalizes with VEGF binding molecules. Analysis of fluorescence recovery after photobleaching reveals that binding/unbinding to matrix molecules dominates VEGF dynamics in the pericellular region. Computational simulations using our experimental measurements of kinetic parameters show that matrix retention of chemotactic signals can lead to the formation of reticular cellular networks on a realistic timescale. Taken together, these results show that VEGF binds to matrix molecules in proximity of HUVECs in Matrigel, and suggest that bound VEGF drives vascular network patterning.
The effects of temperature and sliding speed on the tribological behavior of a SIC ceramic by sliding on the same material in deoxygenated water were investigated from room temperature to 300°C under the corresponding saturated vapor pressures. The friction coefficient and specific wear rates of both plates and disks increased at elevated temperatures at all sliding speeds, but decreased with increasing sliding speed at 120" and 300°C. Fine mirrorlike worn surfaces were observed without wear debris under all sliding conditions. The wear mechanism appears to consist of hydrothermal oxidation of SIC and dissolution of reaction products such as silica.
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