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When tryptophan is glycated with glucose, it results in forming two types of glycated tryptophan compounds, glucose-tryptophan Amadori product and (1R, 3S) -1-(D-gluco-1, 2, 3, 4, 5-pentahydroxypentyl) -1, 2, 3, 4-tetrahydro-β-carboline-3-carboxylic acid (PHP-THβC). As hyperglycemia and high body temperature are the characteristics in avian species, these are supposed to elevate the concentration of glycated tryptophan compounds in the plasma of chickens. However, there was no attempt to detect two types of glycated tryptophan compounds in the plasma of chickens, so far. Therefore, young chickens were fed tryptophan-excess diets (0, 1, 2 or 3% excess) for 14 days, and glycated tryptophan compounds were detected using a liquid chromatograph mass spectrometer (LC/MS). In the present study, two types of glycated tryptophan compounds, glucose-tryptophan Amadori product and PHP-THβC, were successfully detected in the plasma of chickens, and plasma levels of both glycated tryptophan compounds significantly correlated to plasma tryptophan concentration.

When tryptophan is glycated with glucose, it results in forming two types of glycated tryptophan, glucosetryptophan Amadori product and (1R, 3S)-1-(D-gluco-1,2,3,4,5-pentahydroxypentyl)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (PHP-THβC). Hyperglycemia and high body temperature in avian species are supposed to elevate the concentration of glycated tryptophan in the plasma of chickens fed tryptophan-excess diets. However, plasma concentrations of both glucose-tryptophan Amadori product and PHP-THβC have not been determined so far. Young chickens were fed tryptophan-excess diets (1, 2 or 3% excess) for 14 or 21 days, and plasma concentrations of two types of glycated tryptophan were measured using liquid chromatograph mass spectrometer (LC/MS). In the present study, we successfully detected and quantified both glucose-tryptophan Amadori product and PHP-THβC in the plasma, which revealed that plasma concentrations of glucose-tryptophan Amadori product and PHP-THβC were approximately 1 and 2-4 μM, respectively. Furthermore, plasma concentrations of glycated tryptophan compounds significantly correlated to that of plasma tryptophans, and total amount of both glycated tryptophan compounds was almost 10% of plasma tryptophan.

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