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An about, 4-fold H2 obtained with a mixed culture of Chlamydomonasreinhardtii and Rhodosprillum rubrum, compared with in the case of an algal culture of C. reinhardtii alone. This increasing effect was due to the consumption of formate formed by C. reinhardtii; R. rubrum evolved hydrogen from formate via the formate hydrogen-lyase system under dark anaerobic (N2) conditions. Maximum H2 evolution by the mixed culture was observed with a ratio of 8 : 2 (alga : bacterium) at a total cell concentration of above 0.6mg dry wt/ml. Sustained H2 production with an alternating light/dark cycle in a membrane reactor, in which this alga and bacterium were cultured in separate compartments, was performed for one week.

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