The objective of this study was to evaluate the effect of copper alone and in combination with lactic acid against E. coli O157:H7 in laboratory medium and on the surface of lettuce and tomatoes. Four strains of E. coli O157:H7 were individually inoculated into BHI broth containing different concentrations of copper (5, 10, 20, and 40 ppm, w/v), lactic acid (0.1 and 0.2%, v/v), and their combinations. After incubation, aliquots of 1 mL from each sample were withdrawn and plated on BHI agar to determine the bacterial population. Significant growth inhibition (P < 0.05) was observed with a combination treatment of copper (40 ppm) and lactic acid (0.2%). The population of E. coli O157:H7 was reduced by 3.93 and 3.39 log on the surface of lettuce and tomato samples, respectively, when treated with the same combination. This indicates that combination of copper and lactic acid could be used as an effective solution to inhibit E. coli O157:H7 on fresh produce.
BackgroundLung cancer consider as one of leading cause of death around the world, normally the disease detection is difficult in early stages, Anew methods for diagnostic using non‐invasive way are developed using Nano‐molecules called exosomes. Exosomes are cell derived vesicles carrying different kind of molecules (proteins, DNA, RNA, microRNA) and also, displaying many proteins on their membrane surfaces. Those exosomes molecules are identical of parental cancer cells that induce them.MethodsIn this study, exosomes were isolated and characterized using multiple centrifugations, ultrafiltration, and chemical precipitation techniques. The resulted products were captured by two electro‐Microscopes (EM) methods for characterizing the morphology of exosomes as to differentiate exosomes from other extracellular vesicles Besides, the resulted products also further characterized by blotting the proteins extracted from exosomes against different monoclonal antibodies (CD81, CD63, CD9) that confirmed the existence of ligands attached to exosomes surfaces in western blot test.Results and Discussionthe exosomes isolated products were screened under Transmission Electron Microscopy (TEM). The size distributions of extracellular vesicles were significantly different from exosomes 160 nm P < 0.001). The size distributions of apoptotic vesicles (APV) 450nm and necrotic bodies, (NCB) 280 nm were significantly different from exosomes (P < 0.001). The same size band was observed in PVDF membranes of western blot test in all three methods using each monoclonal antibody. However, the filtration devices give higher protein yield and greater purity.In ConclusionsThe current results confirmed the accessible use of exosomes as biomarker to detect lung cancer cells.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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