<p><em>Abstrak</em><strong> - Sungai Ciliwung merupakan salah satu sumber kehidupan bagi masyarakat. Berdasarkan survey yang dilakukan, ikan sapu-sapu sungai Ciliwung juga dimanfaatkan sebagai pangan oleh masyarakat. Penelitian ini bertujuan untuk mendeteksi keberadaan bakteri Coliform dan mikroorganisme yang terdapat pada ikan sapu-sapu asal perairan sungai Ciliwung. Deteksi Coliform dilakukan dengan metode uji praduga dan uji konfirmasi terhadap insang, usus, daging serta kulit abdomen ikan sapu-sapu. Uji praduga dengan menggunakan media Lactose Broth (LB), sedangkan uji konfirmasi dengan media Brilliant Lactose Broth (BGLB). Hasil MPN dilihat dari tabel yang memberikan nilai duga terdekat dengan kombinasi tabung positif dan tabung negatif pada uji konfirmasi. Hasil yang didapat menunjukan seluruh sampel memiliki nilai MPN melebihi batas maksimum Coliform pada makanan. Jadi daging, insang, kulit abdomen, dan usus pada ikan sapu-sapu tidak layak untuk dikonsumsi oleh masyarakat.</strong></p><p><em><strong>Kata Kunci</strong> - Coliform, ikan sapu-sapu, sungai Ciliwung</em></p><p><em>Abstract</em><strong> - Ciliwung River is one of life source for society. Based on the survey conducted, Ciliwung river broom fish is also used as food by the community. This study aims to detect the presence of Coliform bacteria and microorganisms found in broom fish from the Ciliwung River waters. Coliform detection was performed by presumptive test method and confirmation test on gills, intestines, meat and abdominal skin of sweeper fish. Prediction test using Lactose Broth (LB) media, while confirmation test with Brilliant Lactose Broth (BGLB) media. MPN results are seen from the table that gives the closest possible value to the positive tube and negative tube combination in the confirmation test. The results show that all samples have MPN values exceeding the maximum limit of Coliform in foods. So the meat, the gills, the abdominal skin, and the intestines in the broom fish are not fit for consumption by the public.</strong></p><p><em>Keywords</em> - Coliform, Cattle Fish, River Cilliwung</p>
Darna bradleyi Holloway, D. trima Moore, Setothosea asigna van Eecke and Setora nitens Walker are sympatric and coseasonal limacodid moths in plantations of oil palm, Elaeis guineensis Jacq. (Arecales: Palmae), in Borneo, southeast Asia. We tested the hypothesis that these four species maintain reproductive isolation through specificity in diel periods of communication, microlocation for communication and/or communication signal (pheromone). Studying diel periodicity of calling behavior by female moths and response by male moths to traps baited with virgin females or synthetic pheromone, we determined that sexual communication of D. bradleyi and D. trima took place from 17:30 to 18:45 hr and that of S. asigna and S. nitens from 18:45 to 20:00 hr and from 18:30 to 19:30 hr, respectively. Over 80% of male S. asigna and S. nitens were captured in pheromone-baited traps suspended \ 5 m high, whereas male D. bradleyi and D. trima were captured mostly in traps B 5 m high. Synthetic pheromone baits attracted male moths in a species-specific manner. Moreover, baits containing both S. asigna and S. nitens pheromones failed to attract any male moths, indicating that female S. asigna and S. nitens, with overlapping communication periods, use bifunctional pheromone components that attract conspecific males while repelling heterospecifics. Similarly, addition of D. bradleyi pheromone to S. asigna or S. nitens pheromone reduced attraction of male S. asigna and S. nitens. The failure of D. bradleyi and D. trima, which overlap in time and microlocation for communication, to evolve bifunctional pheromones may be attributed to the recent occurrence of sympatry between D. bradleyi and D. trima in Borneo, apparently too recent for bifunctional pheromones to have evolved. We conclude that D. bradleyi, D. trima, S. asigna and S. nitens utilize any or all of diel periodicity, intra and interspecific effects of communication signal and/or microlocation for signaling, allowing these limacodids to co-inhabit the same habitat and remain reproductively isolated.
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