Purpose: We assessed whether perioperative circulating tumor DNA (ctDNA) could be a biomarker for early detection of molecular residual disease (MRD) and prediction of postoperative relapse in resected non–small cell lung cancer (NSCLC). Experimental Design: Based on our prospective, multicenter cohort on dynamic monitoring of ctDNA in lung cancer surgery patients (LUNGCA), we enrolled 950 plasma samples obtained at three perioperative time points (before surgery, 3 days and 1 month after surgery) of 330 stage I–III NSCLC patients (LUNGCA-1), as a part of the LUNGCA cohort. Using a customized 769-gene panel, somatic mutations in tumor tissues and plasma samples were identified with next-generation sequencing and utilized for ctDNA-based MRD analysis. Results: Preoperative ctDNA positivity was associated with lower recurrence-free survival (RFS; HR = 4.2; P < 0.001). The presence of MRD (ctDNA positivity at postoperative 3 days and/or 1 month) was a strong predictor for disease relapse (HR = 11.1; P < 0.001). ctDNA-based MRD had a higher relative contribution to RFS prediction than all clinicopathologic variables such as the TNM stage. Furthermore, MRD-positive patients who received adjuvant therapies had improved RFS over those not receiving adjuvant therapy (HR = 0.3; P = 0.008), whereas MRD-negative patients receiving adjuvant therapies had lower RFS than their counterparts without adjuvant therapy (HR = 3.1; P < 0.001). After adjusting for clinicopathologic variables, whether receiving adjuvant therapies remained an independent factor for RFS in the MRD-positive population (P = 0.002) but not in the MRD-negative population (P = 0.283). Conclusions: Perioperative ctDNA analysis is effective in early detection of MRD and relapse risk stratification of NSCLC, and hence could benefit NSCLC patient management.

The reductive dechlorination of pentachlorophenol (PCP) by Geobacter sulfurreducens in the presence of different biochars was investigated to understand how biochars affect the bioreduction of environmental contaminants. The results indicated that biochars significantly accelerate electron transfer from cells to PCP, thus enhancing reductive dechlorination. The promotion effects of biochar (as high as 24-fold) in this process depend on its electron exchange capacity (EEC) and electrical conductivity (EC). A kinetic model revealed that the surface redox-active moieties (RAMs) and EC of biochar (900 °C) contributed to 56% and 41% of the biodegradation rate, respectively. This work demonstrates that biochars are efficient electron mediators for the dechlorination of PCP and that both the EC and RAMs of biochars play important roles in the electron transfer process.

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Microbial fuel cells (MFCs) are a promising technology to recover electrical energy from different types of waste. However, the power density of MFCs for practical applications is limited by the anode performance, mainly resulting from low bacterial loading capacity and low extracellular electron transfer (EET) efficiency. In this study, an open three-dimensional (3D) structured electrode was fabricated using a natural loofah sponge as the precursor material. The loofah sponge was directly converted into a continuous 3D macroporous carbon material via a simple carbonization procedure. The loofah sponge carbon (LSC) was decorated with nitrogen-enriched carbon nanoparticles by cocarbonizing polyaniline-hybridized loofah sponges to improve their microscopic structures. The macroscale porous structure of the LSCs greatly increased the bacterial loading capacity. The microscale coating of carbon nanoparticles favored EET due to the enhanced interaction between the bacteria and the anode. By using a single-chamber MFC equipped with the fabricated anode, a power density of 1090 ± 72 mW m(-2) was achieved, which is much greater than that obtained by similarly sized traditional 3D anodes. This study introduces a promising method for the fabrication of high-performance anodes from low-cost, sustainable natural materials.

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