In this paper, the mechanical properties of erythrocytes were studied numerically based upon the mechanical model originally developed by Pamplona and Calladine (ASME J. Biomech. Eng., 115, p. 149, 1993) for liposomes. The case under study is the erythrocyte stretched by a pair of laser beams in opposite directions within buffer solutions. The study aims to elucidate the effect of radiation pressure from the optical laser because up to now little is known about its influence on the cell deformation. Following an earlier study by Guck et al. (Phys. Rev. Lett., 84, p. 5451, 2000; Biophys. J., 81, p. 767, 2001), the empirical results of the radiation pressure were introduced and imposed on the cell surface to simulate the real experimental situation. In addition, an algorithm is specially designed to implement the simulation. For better understanding of the radiation pressure on the cell deformation, a large number of simulations were conducted for different properties of cell membrane. Results are first discussed parametrically and then evaluated by comparing with the experimental data reported by Guck et al. An optimization approach through minimizing the errors between experimental and numerical data is used to determine the optimal values of membrane properties. The results showed that an average shear stiffness around 4.611x10-6 Nm(-1), when the nondimensional ratio of shear modulus to bending modulus ranges from 10 to 300. These values are in a good agreement with those reported in literature.
The prevention and control of navel orange pests and diseases is an important measure to ensure the yield of navel oranges. Aiming at the problems of slow speed, strong subjectivity, high requirements for professional knowledge required, and high identification costs in the identification methods of navel orange pests and diseases, this paper proposes a method based on DenseNet and attention. The power mechanism fusion (DCPSNET) identification method of navel orange diseases and pests improves the traditional deep dense network DenseNet model to realize accurate and efficient identification of navel orange diseases and pests. Due to the difficulty in collecting data of navel orange pests and diseases, this article uses image enhancement technology to expand. The experimental results show that, in the case of small samples, compared with the traditional model, the DCPSNET model can accurately identify different types of navel orange diseases and pests images and the accuracy of identifying six types of navel orange diseases and pests on the test set is as high as 96.90%. The method proposed in this paper has high recognition accuracy, realizes the intelligent recognition of navel orange diseases and pests, and also provides a way for high-precision recognition of small sample data sets.
proliferation after treated with lactoferrin (10 mmol/L) for 24 hours and 48 hours respectively, and the results of western blot showed that LTF inhibited proliferation of nasopharyngeal carcinoma cells in vitro by modulating the mitogen-activated protein kinase pathway.The function of the human bronchial epithelial cells (HBECs) is to act as barriers which can protect the underlying tissue against allergens, irritants, viruses, and microbial pathogens. The effect of the barriers relies on epithelial integrity which in turn is dependent on intercellular adhesion. Epithelial cadherin (E-cd) is an important adhesion molecule leading to adhesion between HBECs. Neuropeptide is constitutively expressed in normal lungs where it localizes to neuroendocrine cells. Among lung neuropeptides, Calcitonin gene-related peptide (CGRP) plays a prominent role for its multiple effects on tissue repair and anti-inflammatory actions. The objective of this study was to investigate the effects of CGRP on E-cd expression of HBECs in vitro. The experiments included following steps. First, the influence of CGRP on E-cd protein and mRNA expression were determined in both unstimulated and O 3 -stressed HBECs using immunocytochemistry and RT-PCR analysis. Second, the signal transduction pathways of CGRP were observed by using PKC inhibitor H-7, calmodulin inhibitor W-7 and PKA inhibitor H-89. The results showed that E-cd was distributed on HBECs. Furthermore, O 3 -stress decreased the membranous E-cd expression but increased the cytoplasm E-cd expression. We propose that the change of E-cd expression might mediate HBECs injuries induced by O 3 -stress and CGRP produced a dose and time dependent increase of membranous E-cd and E-cd mRNA expression on both unstimulated and O 3 -stressed HBECs probably via PKA, PKC and CaM pathways.
Based on the design theory of non-circular gears, using Pro/E drawing module, a system of elliptical gears parametric module and simulation was developed. Machining non-circular gear by wire cutting can be achieved by using Pro / E manufacturing module, and then gear machining tool path is created, tool and process parameters is set and simulation of process tool and process of post-processing analysis was described. Through the above process ultimately to generate post-processing procedures which NC machine tool need and achieve the automatic programming of non-circular gear. Form the application example, it has been observed that the gear created by Pro/E simulated NC programs can correct mesh according to the theory transmission ratio and the effectiveness of the proposed approach.
In order to improve the pneumatic gauging precision of honing process, according to the pneumatic gauging characteristic of honing machine, the mathematical model of air current state in measurement process is established with oblique shock wave theory. Through setting up different fillet on protection plate, the air flow state of measurement process could be improved. The change of gas velocity, temperature and pressure was analyzed for different protection plate structure by fluid simulation software, the validity of the theoretical analysis was verified, which can provide the theory basis for structure optimization of the honing head.
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