3 Japan Collection of A p-nitrophenol-degrading bacterial strain was isolated from industrial wastewater. This strain (NSP413 was identified as a member of the genus Nocardioides from c hemotaxonom i c characterizations and p h y I og enet ic inference based on 165 rDNA sequence analysis. The G+C content is 71.4 mol%.The diamino acid in the cell-wall peptidoglycan is u-diaminopimelic acid. The predominant menaquinone is MK=8(HJ. The cellular fatty acid profile is similar to those of Nocardioides species. 165 rDNA sequence analysis show that strain NSP41T is the most related to Nocardioides simplex strains with a level of nucleotide similarity of 986%. The levels of 165 rDNA similarity between strain NSP4V and other Nocardioides species ranged from 93.8 to 95.1 %. This organism is distinguishable from some other Nocardioides species as well as N.simplex strains by DNA-DNA relatedness data. This organism is different from N. simplex strains in some phenotypic characteristics. Therefore, on the basis of the data presented, a new species of the genus Nocardioides, Nocardioides nitrophenolicus, is proposed. The type strain of the new species i s strain NSP41T (= KCTC 0457BP3.
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