An experiment was performed in a completely randomized split-plot design using five lines of spring wheat (Triticum aestivum L.) (AW-774, AC Carberry, HY-162, Major and AAC Scotia) and two arbuscular mycorrhizal fungi (AMF) strains (Rhizoglomus irregulare and Glomus cubense). Two different inoculant forms (solid and liquid) for the G. cubense strain were evaluated. The main plot was AMF, and the subplot was spring wheat lines. Data on heading date, plant height, fresh, and dry biomass, yield, grain quality (chemical composition of the seeds, gluten, and sugar), root structure, and colonization by AMF were collected. The results show a positive effect of inoculation in comparison with the control treatment. The liquid and solid G. cubense inoculants provided better results than inoculation with R. irregulare. Fungus indicators were in agreement with root morphological parameters because of the effect induced by AMF activity. Yield increased significantly in the mycorrhizal treatments.