The present study shows that natural killer cell-mediated cytotoxicity of BALB/c mouse spleen cells to syngeneic tumor cells was augmented by in vivo priming or in vitro stimulation with the streptococcal preparation OK432. The augmentation of spleen cell cytotoxicity to syngeneic tumor cells by in vivo priming alone with OK432 was lower than that obtained by in vitro stimulation alone with OK432. When the murine spleen cells primed in vivo with OK432 were rechallenged in vitro with OK432 at various intervals, the natural cytotoxicity was more strongly enhanced than that seen with in vitro stimulation alone. The cell surface phenotype of killer cells activated with OK432 was Thy 1+ and asialo GM1+, suggesting the activated natural killer cell. Next, mice were transplanted with syngeneic colon adenocarcinoma cells, and primed in vivo with OK432. These spleen cells were subsequently challenged in vitro with OK432. These spleen cells displayed a strong cytotoxic activity not only to the transplanted adenocarcinoma cells but also to other syngeneic tumor cells.
Background: Technetium (Tc) 99m methoxyisobutyl isonitrile (qqmTc-MIBI) has recently been introduced for parathyroid imaging, as well as for myocardial imaging. We studied the usefulness of Y9n7Tc-MIBI scintigraphy for preoperative localization of abnormal parathyroid glands. Methods: The usefulness of yqmTc-MIBI scintigraphy for detection of hyperfunctional parathyroid lesions was evaluated in 5 patients with primary hyperparathyroidism. The results of localizing the abnormal glands by using qqmTc-MIBI were compared with those obtained by using thallium (TI) 201 -technetium (Tc) 99m (zulTI-yqmTc) subtraction scintigraphy, computed tomography, and ultrasonography. Results: The delayed (2 hours) imaging of ygmTc-MIBI scintigraphy was highly useful for accurate localization of the abnormal parathyroid lesions. The diseased glands were detected in all cases where gqmTc-MIBI scintigraphy was used, and using q9mTc-MIBI scintigraphy provided more information than did computed tomography, ultrasonography, or 2n1Tl-ygmTc subtraction scintigraphy. Conclusion: This method is simple and essential for detecting hyperfunctioning parathyroid glands, especially those with small or ectopic lesions. This technique should be widely applied as a localizing diagnostic method for hyperparathyroidism.
Int
We have performed hepatectomy using microwave tissue coagulator which we have developed in about 122 patients with liver cancer associated with cirrhosis. Merit of this high hemostatic performance has been put to use from the first and operations with this have been considered to be those which hardly require transfusion during operation. We introduce you the keys and points to be regarded of recent operative technique. Points in operation can be summarized as follows : 1. Portal blocking is not made, 2. Cholecystectomy is made and a catheter is placed in the cystic duct, 3. The line for incision is determined previously by ultrasonography, 4. Coagulation is made mimutely, strongly and in no hurry(80W. 45s), 5. A line for incision is determined to leave coagulation zone which extends from the electrode impaling site to 10 mm thereof sufficiently on the side of the remaining liver. 6. Coagulation in the neighborhood of large vessels is avoided, 7. Even though cutting suture occurred at the ligature of small vessel systems, Z-type ligature is applied afterward, 8. When the resection is finished the absence of stump bleeding is confirmed and then indigocamine is infused from a tube which is placed previously and biliary leakage test is made without fail, 9. Omentopexy is made without fail to remove a dead space between the subdiaphragmatic surface and liver. 10. The drain is removed as early as possible to prevent retrograde infection.
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