In recent decades, hybrid imaging techniques that exploit the advantages of multiple imaging technologies have aroused extensive attention due to the deficiencies of single imaging modes. Along with the development of single photon emission computed tomography-magnetic resonance imaging (SPECT-MRI), it is currently necessary to develop a series of dual probes that can combine the outstanding sensitivity of SPECT with the high spatial resolution of MRI. Herein, the commonly used technetium-99 (Tc) was labelled on the surface of manganese oxide-based mesoporous silica nanoparticles (MnO-MSNs) for use in SPECT-MRI dual-modal imaging. The radiolabelling yield was as high as 99.1 ± 0.6%, and the r value of the nanoprobes was able to reach 6.60 mM s due to the pH-responsive properties of the MnO-MSNs. The high-performance SPECT-MRI dual-modal imaging was confirmed in vivo in tumour-bearing mice, which could also provide semi-quantitative information for tumour detection. Importantly, these nanoprobes can deliver anti-cancer drugs in cancer therapy due to their unique mesoporous structures. Thus, nanotheranostics combining dual-modal imaging with anti-cancer therapeutic properties were achieved.

Over the last three years, the dockless bike sharing scheme has become prevalent in the context of the boom in the sharing economy, the wide use of mobile online payment, the increasing environmental awareness and the inherent market demand. This research takes Beijing as a case study, investigates the users’ characteristics, their behaviour change, and perceptions of dockless bike sharing scheme by the quantitative survey, and then analyzes the reasons behind it and how it has changed the residents’ life in Beijing. This new kind of dockless shared bikes, with great advantages of accessibility, flexibility, efficiency and affordability, helps to solve the ‘last mile’ problem, reduce the travel time, and seems to be very environmentally-friendly and sustainable. However, with the help of interview and document analysis, this research finds that the shared bikes are not the effective alternative for the frequent car-users. Nevertheless, it also has numerous negative consequences such as ‘zombie’ bikes blocking the sidewalks and vandalism to the bikes. The public is also worried about their quality and safety, especially the issues of ‘right of way’. How to coordinate and solve these problems is not only related to the future direction of the dockless bike sharing scheme but also to the vital interests of the general public. Therefore, it is important to emphasize that governments, enterprises, and the public participate in multi-party cooperation and build synergic governance networks to carry forward the advantages and avoid the negative effects of the new bike sharing system.

We previously demonstrated that proline-rich protein 11 (PRR11) and spindle and kinetochore associated 2 (SKA2) constituted a head-to-head gene pair driven by a prototypical bidirectional promoter. This gene pair synergistically promoted the development of non-small cell lung cancer. However, the signaling pathways leading to the ectopic expression of this gene pair remains obscure. In the present study, we first analyzed the lung squamous cell carcinoma (LSCC) relevant RNA sequencing data from The Cancer Genome Atlas (TCGA) database using the correlation analysis of gene expression and gene set enrichment analysis (GSEA), which revealed that the PRR11-SKA2 correlated gene list highly resembled the Hedgehog (Hh) pathway activation-related gene set. Subsequently, GLI1/2 inhibitor GANT-61 or GLI1/2-siRNA inhibited the Hh pathway of LSCC cells, concomitantly decreasing the expression levels of PRR11 and SKA2. Furthermore, the mRNA expression profile of LSCC cells treated with GANT-61 was detected using RNA sequencing, displaying 397 differentially expressed genes (203 upregulated genes and 194 downregulated genes). Out of them, one gene set, including BIRC5, NCAPG, CCNB2, and BUB1, was involved in cell division and interacted with both PRR11 and SKA2. These genes were verified as the downregulated genes via RT-PCR and their high expression significantly correlated with the shorter overall survival of LSCC patients. Taken together, our results indicate that GLI1/2 mediates the expression of the PRR11-SKA2-centric gene set that serves as an unfavorable prognostic indicator for LSCC patients, potentializing new combinatorial diagnostic and therapeutic strategies in LSCC.