Adaptin ear-binding coat-associated protein 2 (NECAP2) belongs to the family of proteins encoding adaptin-ear-binding coat-associated proteins. However, its immune effect on tumors and its microenvironment are still unclear. Here, we systematically evaluated the differences (variations) in NECAP2 expression for low-grade glioma (LGG) and pan-cancer in the LGG dataset of The Cancer Genome Atlas (TCGA) utilizing bioinformatics methods. We found for the first time that NECAP2 level was elevated in gliomas and that this upregulation increased as the tumor grade increased. In addition, Pearson correlations of NECAP2 with five immune pathways and significant gene mutations associated with NECAP2 were also analyzed. Univariate survival and multivariate Cox analyses were used to compare the clinical characteristics and survival of the patients. Glioma patients with NECAP2 overexpression have a remarkably higher risk of developing malignant behavior and a worse prognosis. The correlation between the expression levels of NECAP2 and the prognosis of glioma patients was identified. Kaplan-Meier curves showed that patients with upregulated NECAP2 expression exhibited an unfavorable prognosis. Western blotting showed that NECAP2 was overexpressed in glioma patients. IHC staining results illustrated an elevation in the NECAP2 protein expression level with the development of tumor malignancy. Additionally, qRT-PCR verified that oxidative stress in glioma tissues reduced the expression of stress-related genes and oxidative stress capacity compared to normal tissues, which may be associated with tumor evasion of immune surveillance and tumor progression. In vitro wound-healing and Transwell assay confirmed that NECAP2 promotes glioma cell migration and invasion. Our study also thoroughly examined the immune significance of NECAP2 in the TCGA-LGG samples, using CIBERSORT and ESTIMATE to explore the correlation between NECAP2 and cancer immune infiltration. The NECAP2 expression levels were correlated with the infiltration degree of immune cells such as neutrophils, CD4+ T cells, macrophages, CD8+ T cells, and B cells. Therefore, our results indicate that NECAP2 strongly correlates with the overall immune infiltration level of glioma and could independently serve as a prognostic biological marker for glioma patients.
The purpose of this study was to explore the relationship between total physical activity and different dimensions of cognitive function (orientation, attention, and memory) among older adults in rural Sichuan, China. This cross-sectional study involved 715 older adults (average age of 72 years). Total PA was measured by the Physical Activity Scale for the Elderly, and cognitive function was assessed by the Telephone Interview for Cognitive Status (TICS) questionnaire. The multivariate linear regression analysis indicated that total PA and household PA were significantly associated with the overall Telephone Interview for Cognitive Status score (β = 0.143, p < .001; β = 0.115, p = .002, respectively), the orientation dimension (β = 0.142, p < .001; β = 0.131, p = .001, respectively), and the memory dimension (β = 0.179, p < .001; β = 0.134, p = .001, respectively). The study showed a positive association between total PA, household PA, and cognitive function in older adults, especially in the orientation dimension and the memory dimension of cognitive function.
785 middle and high school students were polled using the Parent-Child Relationship Scale, the Adolescent Risk-taking Behavior Questionnaire, and the School Connectedness Scale in order to examine the mechanisms underlying the parent-child relationship's influence on adolescent risk-taking action. The findings revealed that (1) parent-child relationship had a considerably negative effect on adolescent risk-taking behavior; (2) School connectedness was a significant mediating variable of parent-child relationship influencing adolescent risk-taking behavior. The findings revealed that both parent-child relationship and school connectedness are significant predictors of adolescent risk-taking behavior, and parent-child relationship can influence adolescent risk-taking behavior by affecting school connectedness. This study has certain guiding significance for the intervention of adolescent risk-taking behavior.
Fiber- and yarn-based thermoelectric materials play an essential role in the design of fabric-based flexible thermoelectric generators which may overcome the wearable difficulties of existing film-based flexible thermoelectric generators. In this study, we used a robust coating method to produce high-performance thermoelectric yarns for wearable applications. An organic/inorganic hybrid coating agent composed of PEDOT:PSS, MWCNT, and Bi2Te3 was used to coat an alkali modified porous polyester yarn. The organic/inorganic hybrid material contributes to the improved thermoelectric properties. The porous modification of polyester yarns improves the wicking property of the fibers and enhances the adhesion stability between yarn substrate and the coating layer. A compromised optimal power factor of 12.3 μWm-1K-2 could be achieved by 20 wt% Bi2Te3 loading. The corresponding electrical conductivity and Seebeck coefficient were 5526.8 S/m and 47.1 μV/K at room temperature respectively. A fabric thermoelectric generator with five yarn legs could generate an open circuit voltage of 2.95 mV at a temperature difference of 30 ℃, demonstrating its potential application in wearable applications.
AimsThe aim of this study was to evaluate the effect of epicatechin, on neurological recovery and neuroinflammation after traumatic brain injury (TBI) to investigate its potential value in clinical practice.MethodsTBI model was established in adult rats by CCI method. The effect of epicatechin was evaluated after intraperitoneal injection. Neurological recovery after TBI was assessed by Morris Water Maze, mNSS score, Rotarod test and Adhesive removal test. Protein and gene expression was assessed by Western blot, ELISA, PCR and immunofluorescence. Furthermore, the use of AKT pathway inhibitors blocked the therapeutic effects of epicatechin clarifying AKT‐P53/CREB as a potential pathway for the effects of epicatechin.ResultsAdministering epicatechin after TBI prevented neuronal death, reduced neuroinflammation, and promoted neurological function restoration in TBI rats. Network pharmacology study suggested that epicatechin may exert its therapeutic benefits through the AKT‐P53/CREB pathwayConclusionThese results indicate that epicatechin, a monomeric compound derived from tea polyphenols, possesses potent antioxidant and anti‐inflammatory properties after TBI. The mechanism may be related to the regulation of the AKT‐P53/CREB signal pathway.
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