In this study, we sequenced the first full-length insect transcriptome using the Erthesina fullo Thunberg based on the PacBio platform. We constructed the first quantitative transcription map of animal mitochondrial genomes and built a straightforward and concise methodology to investigate mitochondrial gene transcription, RNA processing, mRNA maturation and several other related topics. Most of the results were consistent with the previous studies, while to the best of our knowledge some findings were reported for the first time in this study. The new findings included the high levels of mitochondrial gene expression, the 3 0 polyadenylation and possible 5 0 m 7 G caps of rRNAs, the isoform diversity of 12S rRNA, the polycistronic transcripts and natural antisense transcripts of mitochondrial genes et al. These findings could challenge and enrich fundamental concepts of mitochondrial gene transcription and RNA processing, particularly of the rRNA primary (sequence) structure. The methodology constructed in this study can also be used to study gene expression or RNA processing of nuclear genomes.
The flourish of nanotechnology has brought new vitality to the research and development of electrochemical sensing materials. In this work, we successfully synthesized Nano Au and Pt alloy microspheres decorated with reduced graphene oxide (RGO/nAPAMSs) by a simple, facile, and eco-friendly one-step reduction strategy for the fabrication of highly sensitive nonenzymatic HO sensing interfaces. Energy-dispersive X-ray spectroscopy mapping (EDX mapping), energy-dispersive X-ray spectroscopy analysis (EDX), transmission electron microscopy (TEM), Fourier transform infrared spectrum (FT-IR), and X-ray diffraction spectrum (XRD) were employed to characterize RGO/nAPAMSs from a microscopic perspective. The results of cyclic voltammetry and chronoamperometry exhibited excellent electrochemical behaviors toward HO, with a rapid response time within 5 s, remarkable sensitivity of 1117.0 μA mM cm, wide linear range of 0.005 to 4.0 mM and lower detection limit of 0.008 μM (S/N = 3), which provide RGO/nAPAMS not only a promising prospect for the quantitative detection of HO but also a potential application in other fields of sensors. Moreover, further analysis showed the principles of the superior HO sensing performance of RGO/nAPAMSs. This discovery provides a significant contribution to future study in nonenzymatic HO sensing based on Nano Pt, Nano Au noble metal electrocatalysts.
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