Ubiquitin (Ub)-based fluorescent
reagents are crucial to explore
the activity of deubiquitinases (DUBs). Ub-Rho110-G is one of the
preferred tools, whereas the current synthetic route is time-consuming.
Here, we report a new semisynthetic strategy to produce Ub-Rho110-G
through direct aminolysis of Boc-protected Ub-Mesna using bisglycyl-rhodamine
110. We also applied this strategy to synthesize active SUMO2-Rho110-G
for the first time. Biochemical analysis demonstrated that semisynthetic
Ub or SUMO-Rho110-G can be effectively used for the detection of the
activity of DUBs or SUMO-specific enzymes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.