The relationship between osteoblasts and angiogenesis is vital for bone regeneration, especially mandibular and maxillary bones. Transforming growth factor β1 (TGF-β1) and vascular endothelial growth factor (VeGF) are closely related to angiogenesis; however, the regulatory mechanism between them remains unknown. The present study aimed to reveal this mechanism to provide novel insight for development of potential therapeutic opportunities. Western blotting and reverse transcription-quantitative Pcr was used to assess the protein and mrna expression levels in Mc3T3-e1 preosteoblast cells and HuVecs, eliSas were used to detect the expression levels of secreted VeGF, MTT assays were used to assess the viability of the cells, migratory ability was assessed using Transwell assays, angiogenesis assays were used to analyze the formation of blood vessels, and TGF-β1 regulation was confirmed using a dual-luciferase reporter assay. The overexpression of specificity protein 1 (SP1) or TGF-β1 increased VeGF expression levels and secretion, and promoted angiogenesis of co-cultured HuVecs. SP1 also promoted SMad2 phosphorylation. These effects of SP1 were all reversed by the TGF-β1 inhibitor. The VeGF inhibitor bevacizumab also reduced the SP1/TGF-β1/SMad2 pathway-induced angiogenesis of preosteoblasts. in conclusion, it was demonstrated that SP1 promoted TGF-β1 expression, activated the SMad2 pathway and induced VeGF secretion, which may enhance angiogenic processes in preosteoblasts.
Background Micronaire is a comprehensive index reflecting the fineness and maturity of cotton fiber. Micronaire is one of the important internal quality indicators of the cotton fiber and is closely related to the value of the cotton fiber. Understanding the genetic basis of micronaire is required for the genetic improvement of the trait. However, the genetic architecture of micronaire at the genomic level is unclear. The present genome-wide association study (GWAS) aimed to identify the genetic mechanism of the micronaire trait in 83 representative upland cotton lines grown in multiple environments. Results GWAS of micronaire used 83 upland cotton accessions assayed by a Cotton 63 K Illumina Infinium single nucleotide polymorphism (SNP) array. A total of 11 quantitative trait loci (QTLs) for micronaire were detected on 10 chromosomes. These 11 QTLs included 27 identified genes with specific expression patterns. A novel QTL, qFM-A12–1, included 12 significant SNPs, and GhFLA9 was identified as a candidate gene based on haplotype block analysis and on strong and direct linkage disequilibrium between the significantly related SNPs and gene. GhFLA9 was expressed at a high level during secondary wall thickening at 20∼25 days post-anthesis. The expression level of GhFLA9 was significantly higher in the low micronaire line (Msco-12) than that in the high micronaire line (Chuangyou-9). Conclusions This study provides a genetic reference for genetic improvement of cotton fiber micronaire and a foundation for verification of the functions of GhFLA9.
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