The asexual freshwater planarian Dugesia japonica has emerged as a medium-throughput alternative animal model for neurotoxicology. We have previously shown that D. japonica are sensitive to organophosphorus pesticides (OPs) and characterized the in vitro inhibition profile of planarian cholinesterase (DjChE) activity using irreversible and reversible inhibitors. We found that DjChE has intermediate features of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Here, we identify two candidate genes (Djche1 and Djche2) responsible for DjChE activity. Sequence alignment and structural homology modeling with representative vertebrate AChE and BChE sequences confirmed our structural predictions, and show that both DjChE enzymes have intermediate sized catalytic gorges and disrupted peripheral binding sites. Djche1 and Djche2 were both expressed in the planarian nervous system, as anticipated from previous activity staining, but with distinct expression profiles. To dissect how DjChE inhibition affects planarian behavior, we acutely inhibited DjChE activity by exposing animals to either an OP (diazinon) or carbamate (physostigmine) at 1 µM for 4 days. Both inhibitors delayed the reaction of planarians to heat stress. Simultaneous knockdown of both Djche genes by RNAi similarly resulted in a delayed heat stress response. Furthermore, chemical inhibition of DjChE activity increased the worms' ability to adhere to a substrate. However, increased substrate adhesion was not observed in Djche1/Djche2 (RNAi) animals or in inhibitor-treated day 11 regenerates, suggesting this phenotype may be modulated by other mechanisms besides ChE inhibition. Together, our study characterizes DjChE expression and function, providing the basis for future studies in this system to dissect alternative mechanisms of OP toxicity.

The rapid development of perovskite solar cells (PSCs) has stimulated great interest in the fabrication of colorful PSCs to meet the needs of aesthetic purposes in varied applications including building integrated photovoltaics and wearable electronics. However, it remains challenging to prepare high-efficiency PSCs with attractive colors using perovskites with broad optical absorption and large absorption coefficients. Here we show that high-efficiency PSCs exhibiting distinct structural colors can be readily fabricated by employing TiO 2 nanobowl (NB) arrays as a nanostructured electron transport layer to integrate with a thin overlayer of perovskite on the NB arrays. A new crystalline precursor film based on lead acetate was prepared through a Lewis acid-base adduct approach, which allowed for the formation of a uniform overlayer of high-quality CH 3 NH 3 PbI 3 crystals on the inner walls of the NBs. The PSCs fabricated using the TiO 2 NB arrays showed angle-dependent vivid colors under light illumination. The resultant colorful PSCs exhibited a remarkable photovoltaic performance with a champion efficiency up to 16.94% and an average efficiency of 15.47%, which are recordbreaking among the reported colorful PSCs.

Creation of electrons and positrons from light alone is a basic prediction of quantum electrodynamics, but yet to be observed. Our simulations show that the required conditions are achievable using a high-intensity two-beam laser facility and an advanced target design. Dual laser irradiation of a structured target produces high-density γ rays that then create > 108 positrons at intensities of 2 × 1022 Wcm−2. The unique feature of this setup is that the pair creation is primarily driven by the linear Breit-Wheeler process (γγ → e+e−), which dominates over the nonlinear Breit-Wheeler and Bethe-Heitler processes. The favorable scaling with laser intensity of the linear process prompts reconsideration of its neglect in simulation studies and also permits positron jet formation at experimentally feasible intensities. Simulations show that the positrons, confined by a quasistatic plasma magnetic field, may be accelerated by the lasers to energies >200 MeV.

One of the key aspects contributing to the rapid development of perovskite solar cells is to prepare high-quality perovskite films via morphology control and interface engineering. Here, we demonstrate that the additive HPbI 3 works effectively on both morphology control and grain boundary passivation of CH 3 NH 3 PbI 3−x Cl x thin films. By inducing HPbI 3 to the crystal transformation process, high-quality perovskite films consisting of micro-sized grains with boundaries passivated by PbI 2 can be readily produced. The perovskite film obtained with HPbI 3 as the additive achieves a much longer carrier lifetime compared to the pristine perovskite film without the additive. Under the optimal HPbI 3 amount (5.0%), the average power conversion efficiency of the planarheterojunction solar cells is increased by ∼24% to 17.42% from 14.09% for the device without the additive, and the champion efficiency reaches 18.59%. The devices without any encapsulation show impressive shelf stability, retaining more than 85% of the initial efficiency after being stored in ambient environment for over 40 days.

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Creation of electrons and positrons from light alone is a basic prediction of quantum electrodynamics, but yet to be observed. Here we show that it is possible to create >10 8 positrons by dual laser irradiation of a structured plasma target, at intensities of 2 × 10 22 Wcm −2 . In contrast to previous work, the pair creation is primarily driven by the linear Breit-Wheeler process (γγ → e + e − ), not the nonlinear process assumed to be dominant at high intensity, because of the high density of γ rays emitted inside the target. The favorable scaling with laser intensity of the linear process prompts reconsideration of its neglect in simulation studies, but also permits positron jet formation at intensities that are already experimentally feasible. Simulations show that the positrons, confined by a quasistatic plasma magnetic field, may be accelerated by the lasers to energies > 200 MeV.

Based on the combination of Raman spectroscopy with principal component analysis and hierarchical cluster analysis, the molecular mechanism of K562 cell apoptosis induced by Adriamycin in physiological conditions is presented. The obtained results reveal that DNA of K562 cells treated with Adriamycin is lowered greatly, indicating that the damage of the DNA of K562 cells is indeed the main molecular mechanism of K562 cell apoptosis induced by Adriamycin. Specially, by combining principal component analysis and hierarchical cluster analysis, the statistical difference between Raman spectra of different cell types can be revealed effectively. Importantly, this kind of Raman spectroscopy-based multivariate statistical analysis will supply a useful tool for the molecular mechanism detection of cell behavior in physiological conditions.

It has been recently shown that over 109 electron–positron pairs can be produced from light alone at an experimentally accessible laser intensity of [Formula: see text] by irradiating a target with a pre-formed channel by two counter-propagating laser pulses. Although targets of variable length and channel density have been successfully fabricated and used in recent experiments involving high-intensity lasers, the impact of these parameters on the pair yield by different pair creation processes is yet to be understood. In this paper, we explore, using two-dimensional particle-in-cell simulations, the impact of the channel density and length on pair production by the linear Breit–Wheeler process, nonlinear Breit–Wheeler process, and Bethe–Heitler process at fixed laser intensity. We find that these parameters can be successfully used to increase the linear Breit–Wheeler pair yield. More importantly, the relative contribution of each process can be adjusted by varying the same parameters. We show that this approach allows us to completely eliminate the yield from the nonlinear Breit–Wheeler process while maintaining a significant yield from the linear Breit–Wheeler process. The Bethe–Heitler process plays a secondary role in the considered system, so the majority of the positrons inside the channel are produced from light alone. Our results indicate that a structured target irradiated by two laser beams has the potential to be a versatile platform for future experimental studies of the Breit–Wheeler pair production processes, with the target parameters serving as control knobs.