Mitochondrial transcription factor A (TFAM) participates in the process of mitochondrial DNA replication and transcription. microRNAs (miRNAs) serve an important role in the regulation of gene expression. However, the roles of TFAM and certain miRNAs and their associations in the development of numerous cancer types remain unclear. The current study demonstrated that the expression of TFAM was significantly upregulated in colon cancer compared with the normal tissue, while the expression of miRNA-590-3p (miR-590-3p) was predicted with a high score using miRWalk software, and the luciferase assay demonstrated that TFAM was the direct target of miRNA-590-3p. miR-590-3p exhibited high expression levels in both colon cancer tissue and the SW480 cell line. Furthermore, downregulated expression of miR-590-3p significantly inhibited the growth of SW480 cells, which was consistent with results indicating downregulated expression of TFAM in SW480 cells from a previous study. In summary, the results of the current study concluded that miR-590-3p, via direct targeting of TFAM, may serve an important role in the tumorigenesis of colon cancer, and may be a promising target for colon cancer therapeutics.
With the development and generalization of endoscopic technology and screening, clinical application of magnetically controlled capsule gastroscopy (MCCG) has been increasing. In recent years, various types of MCCG are used globally. Therefore, establishing relevant guidelines on MCCG is of great significance. The current guidelines containing 23 statements were established based on clinical evidence and expert opinions, mainly focus on aspects including definition and diagnostic accuracy, application population, technical optimization, inspection process, and quality control of MCCG. The level of evidence and strength of recommendations were evaluated. The guidelines are expected to guide the standardized application and scientific innovation of MCCG for the reference of clinicians.
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