In this study, a hypothetical protein (ORF02740) secreted by Edwardsiella piscicida was identified. We renamed the ORF02740 protein as EvpQ, which is encoded by a mobile genetic element (MGE) in E. piscicida genome. The evpQ gene is spaced by 513 genes from type VI secretion system (T6SS) gene cluster. Low GC content, three tRNA, and three transposase genes nearby evpQ define this MGE that evpQ localizes as a genomic island. Sequence analysis reveals that EvpQ shares a conserved domain of C70 family cysteine protease and shares 23.91% identity with T3SS effector AvrRpt2 of phytopathogenic Erwinia amylovora. Instead, EvpQ of E. piscicida is proved to be secreted at a T6SS-dependent manner, and it can be translocated into host cells. EvpQ is thereof a novel T6SS effector. Significantly decreased competitive index of ΔevpQ strain in blue gourami fish (0.53 ± 0.27 in head kidney and 0.44 ± 0.19 in spleen) indicates that EvpQ contributes to the pathogenesis of E. piscicida. At 8-, 18-, and 24-h post-subculture into DMEM, the transcription of evpQ was found to be negatively regulated by Fur and positively regulated by EsrC, and the steady-state protein levels of EvpQ are negatively controlled by RpoS. Our study lays a foundation for further understanding the pathogenic role of T6SS in edwardsiellosis.
This paper expounds the excellent characteristics of CFRP in engineering application, analyzes the scope of application of CFRP reinforced plastics.This paper introduces the principle and the carbon fiber composite reinforcement method and frame beams and carbon fiber reinforced beam in the structure of the calculation results were analyzed .By SAP2000 software analyzing, results show that after using CFRP reinforced plastics has dramatically enhanced the bearing capacity of the frame beam, improve the ductility of the components, prolong the life span of the structure .
Isolators are included in the new earthquake-resistant standard. This article analyses some questions on base-isolators and presents a non-linear dynamic computing-model for the time-history. A corresponding computer program is also compiled. With the program, the earthquake response of a isolated structure including the displacement, acceleration and the shear force of each floor are calculated and compared with those of the same structure without base-isolation. It has been found that base-isolation has important realistic significance in mitigating earthquake disaster effectively and has enormous economic and social benefits. Therefore it is a kind of extremely popularizing and applying new technology.
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