PURPOSE. To investigate the potential antiproliferative effect of cyclin-dependent kinase inhibitor 1B (CDKN1B) overexpression in a rabbit model of glaucoma filtration surgery (GFS).METHODS. The recombinant adenovector expressing exogenous CDKN1B was delivered to Tenon's capsule by subconjunctival injection during unilateral filtration surgery. The time course of CDKN1B expression was monitored by immunohistochemistry and Western blot analysis. Evaluation of proliferating activity was performed by proliferating cell nuclear antigen (PCNA), argyrophilic nucleolar organizing region (AgNOR) staining, and fibroblast-specific protein 1 (FSP-1). Cyclin-dependent kinase 2 (Cdk2) and Cdk4 expression were detected with immunohistochemical analysis.RESULTS. The overexpression of CDKN1B in Tenon's capsule was monitored throughout the experimental period. Immunoreactivity to CDKN1B was mainly observed in the nucleus of fibroblasts. The increased expression of CDKN1B in sclera was detected up to 21 days after viral infection, whereas the level of CDKN1B protein in corneal stroma was not significantly increased. The overexpression of CDKN1B induced a significant decrease in AgNOR number/nucleus and area/nucleus, PCNA staining, FSP-1 positive cells, and the decreased expressions of Cdk2 and Cdk4, as evidenced by nuclear and cytoplasmic immunoreactivity to Cdk2 and Cdk4 antibodies in positive fibroblasts.CONCLUSIONS. The persistent overexpression of CDKN1B mediated by the recombinant adenovector expressing exogenous CDKN1B in Tenon's fibroblasts after GFS may lead to the inhibition of fibroblast proliferation and the downregulation of Cdk2 and Cdk4 activity, thereby reducing the severity of scar formation and the surgical outcome. (Invest Ophthalmol Vis Sci. 2013;54:343-352)
Ophthalmology is an integral part of general practice education, and it is of great significance to have some knowledge on ophthalmology even if the general practitioner is not an ophthalmologist. The subject characteristics, the traditional mode of teaching, and the cognitive deviation of students have greatly influenced its teaching quality. The application of team-based learning (TBL) in ophthalmology education responds to the demand of modern medical education. Building a team, designing questions that combine theory and clinical practice, as well as employing a fair scoring system can stimulate students’ learning interest and improve their learning autonomy and ability to ask questions and solve problems.
Frosted branch angiitis (FBA) is a rare retinal vascular disease that usually affects healthy, young adolescents and tends to be bilateral. Currently, there are limited reports on FBA worldwide. In this paper, we will present a case of FBA after fever.
Objective: To explore the application effect of the Eyesi surgical simulator in the teaching of cataract surgery for professional ophthalmology postgraduate students. Methods: The professional postgraduate students who were trained in the third year of ophthalmology at the First Affiliated Hospital of Xi’an Medical University were selected as the research objects. After passing the theoretical examination, they were randomly divided into the pig eyeball group, Eyesi group, and pig eye + Eyesi group, with 5 students in each group. The pig eyeball, Eyesi surgery simulator, and pig eye + Eyesi surgery simulator were used for microscopic technique operation and cataract surgery steps training, respectively. After the training, the overall training effects of the three groups of postgraduates were scored, and questionnaires were used to objectively evaluate the three training methods. Results: The scores of the students in the pig eye + Eyesi group were better than those in the Eyesi group, and the students in the Eyesi group were better than those in the pig eyeball group. Conclusion: The Eyesi surgical simulation training system can evaluate the microsurgical skills of professional masters and improve their surgical skills. This system is of great significance for the training of the cataract surgery skills of professional masters.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.