Previous studies have suggested that metformin, a biguanide family member widely used as an oral antidiabetic drug, may inhibit proliferation and induce apoptosis in certain types of cancer cell. However, the molecular mechanisms underlying metformin-associated anticancer effects, and in particular antimetastatic effects, remain to be fully understood. The present study assessed the efficacy of metformin in inhibiting the migration and invasion of the esophageal carcinoma cell line EC109, and evaluated the effect of metformin on the protein kinase B (AKT) signaling pathway. EC109 cells were treated with 0, 5, 10 or 20 mM metformin during the logarithmic growth phase. A Transwell assay and western blot analysis revealed that metformin inhibited the migration and invasion of EC109 cells, nuclear factor-κB activation, matrix metallopeptidase 9 and N-cadherin expression in a phosphorylated-AKT dependent manner. These results suggested that metformin inhibits the migration and invasion of human esophageal carcinoma cells by suppressing AKT phosphorylation and regulating the expression of migration- and invasion-associated genes.
PurposeThe paper aims to study cut resistant property of basic weft plain-knitted fabric for protective clothing.Design/methodology/approachEffects of fiber materials, fabric direction and knitting technology (sinking-depth) were explored, respectively. Cut process of fabric was tracked and the theoretical analysis was provided to evaluate energy transferring of cutting. Fiber-based cut behavior was observed by SEM images. Deformation energy stored in the loop due to yarn bending was regard as initial elastic potential energy of the fabric, which was related to loop structure.FindingsCut resistance of the fiber material was the dominant factor for cut resistance of weft plain-knitted fabric, while unit loop structure played a critical role in improving cut resistance.Social implicationsCut resistance of the fiber material was the dominant factor for cut resistance of weft plain-knitted fabric, while the unit loop structure played a critical role in improving cut resistance.Originality/valueThe paper provides theoretical support of developing flexible protective clothing.
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