Background-Stent-based antiproliferative therapy appears to decrease in-stent restenosis. However, alternative approaches might produce equivalent efficacy with better long-term safety. In previous work, an adenovirus capable of expressing the tissue inhibitor of metalloproteinase-3 (RAdTIMP-3) inhibited neointima formation in cell cultures and porcine saphenous vein grafts. RAdTIMP-3 decreased smooth muscle cell migration, stabilized the extracellular matrix, and uniquely promoted apoptosis. The current study developed eluting stent technology to deliver RAdTIMP-3 during stenting of pig coronary arteries. Methods and Results-Binding of virus to and elution from stents and transduction of pig coronary arteries were confirmed using -galactosidase as a reporter gene in vitro and in vivo. Deployment of RAdTIMP-3-coated stents increased apoptosis and reduced neointimal cell density, but did not increase inflammation or proliferation compared with -galactosidase-expressing adenovirus (RAdlacZ
Purpose: To demonstrate magnetic resonance (MR) measurements of vascular lumen dynamics in common carotid arteries by using true fast imaging with steady-state precession (TrueFISP) cine imaging with an aim to provide additional physiologic information on the vessels. Materials and Methods:The left and right common carotid arteries were studied in normal young men (N ϭ 6; age ϭ 21-24 years; body weight ϭ 130 -175 lbs) using electrocardiogram (ECG)-triggered TrueFISP cine imaging at 20 frames per cardiac cycle. Lumen area waveforms were characterized with specific time and amplitude ratios. Distension values were quantified.Results: Distension values were measured at 25.92 Ϯ 2.58% and 27.58 Ϯ 4.44% for the left and right common carotid arteries, respectively. These findings are consistent with those previously documented using ultrasound imaging in a similar age group. Consistent lumen area waveform characteristics were found among the subjects studied.Conclusion: These findings demonstrate for the first time that the use of TrueFISP cine imaging is a robust, rapid technique for quantifying carotid lumen area dynamics and distension, which may be valuable in characterizing and diagnosing cardiovascular diseases. MAGNETIC RESONANCE IMAGING (MRI) has been used extensively to image the arterial lumen and provide detailed information about vessel wall characteristics. The morphology and composition of the vasculature have been assessed in numerous MR vascular studies. One technique of choice is the turbo spin-echo pulse sequence with the double-inversion recovery approach to better visualize the lumen and vessel wall (1-3), yielding lumen area and wall thickness measurements. MRI has also been used to differentiate individual plaque components for atherosclerosis patients (4,5). Major plaque components can be characterized in T1-, T2-, and intermediate-weighted images (6,7). In cases of aortic aneurysm or suspected aortic dissection, MRI shows promise as a fast, accurate technique for investigating and monitoring the aorta (8).Although MRI is useful for assessing anatomic and certain pathologic properties of the vasculature, it has been used rarely for evaluating the dynamic properties of blood vessels. Data on arterial wall dynamics can reveal how a targeted artery contracts and expands during the cardiac cycle. Such dynamics can reflect the elasticity of blood vessels and thus the functional properties of the vasculature (9,10). Previous studies have shown an association between arterial stiffness and occurrence of cardiovascular diseases, such as hypertension, atherosclerosis, and stroke (11,12). This association arises from the idea that a decrease in arterial elasticity, or a stiffer artery, leads to reduced compliance for storage of blood volume. This contributes to an increased systolic pressure, posing further risk of cardiovascular abnormalities (9). Just as early detection of functional impairment of arteries can improve treatment strategies for preventing cardiovascular diseases (13), evaluating arterial d...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.