Based on the monoclonal antibodies against 3, 3', 5, 5'-tetraiodo-L-thyronine (T 4 ), a 5 competitive indirect chemiluminescence immunoassay (CLEIA) used for quantitative 6 determination of total T 4 in human serum was established. The relevant results indicated that 7 the determined coating antigen concentration was 2.7 µg mL -1 and the CLEIA titer of ascites 8 was 1:8.0×10 4 . The standard curve was 0.495 1.095 y x = − + , with coefficient of 9 determination, R 2 = 0.988,and derived half inhibition concentration (IC 50 ) of T 4 was 16.0 10 ng mL -1 . The system detection limit was 9.23 ng mL -1 . The mean inter-and intra-assay 11 variations were 5.89 % and 4.02 %, respectively. The detected data of total T 4 in human 12 serum from seven patients with this method exhibited significant correlation with reported 13 clinical data, suggesting this CLEIA method could be applicable to clinical diagnosis of 14 thyroid gland disease. 15 16 17 Yilin Gao and Zhirui Deng contributed equally to this work.
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