Background Hyperpigmentation particularly in the cosmetic industry is one of the most important issue that causes excess production and accumulation of melanin. Nowadays, some materials such as kojic acid and its derivatives and arbutin as a skin whitening are used to overcome hyperpigmentation. Since they have side effects such as skin irritation and contact dermatitis, the new surveys focus on agents which are natural and have no side effect.Methods and Results In the present study, we investigated that effect of soy cell culture extract on α-MSH induced melanogenesis in B16-F10 melanoma cells. We found that the soy cell culture extract supressed melanin sythesis and showed anti tyrosinase activity on B16-F10 melanoma cells in a dose dependent manner without cytotoxicity. In addition, we determined that soy cell culture extract suppressed the expression of TYR, TRP1, TRP2 and MITF at both the gene expression and protein level. Conclusions In conclusion, according to the current results, soy cell culture extract has high potential for using as an ingredient in skin whitening products.
This study was a purpose to investigate the anti-aging activity of Comfrey cell suspension culture (CCSC) extract. The effects of CCSC extract on human normal fibroblast cells (CRL-2076) were examined. Comfrey callus and suspension culture were obtained in Murashige and Skoog (MS) medium including 1 mg/L 2,4-Dichlorophenoxyacetic acid (2.4-D). Total phenolic contents were determined in CCSC lyophilizes, the highest phenolic content was found to be 1181 µM gallic acid equivalent in the extract prepared with 70% ethanol solvent. The half-life concentration (IC50 value) of the extract was determined as 83 µg/ml with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. It was found that CCSC extract incubated for 48 h did not reduce cell viability on CRL-2076 cells at doses of 100 and 200 µg/mL. The addition of the CCSC extract at 100 µg/mL concentration to aged CRL-2076 cells increased the collagen type 1 alpha 1 (COL1A1) gene expression level 1.2-fold, elastin (ELN) gene expression level 1.4-fold, and hyaluronan synthase 3 (HAS3) gene expression level 1.4-fold. However, the addition of the CCSC extract at 200 µg/mL concentration to aged CRL-2076 cells increased the COL1A1 gene expression level 1.3-fold, ELN gene expression level 2.7-fold, and HAS3 gene expression level 1.7-fold. In the study, CCSC extract was shown to have anti-aging properties on CRL-2076 cells, suggesting that CCSC extract can be used as active raw material in natural anti-aging cosmetic products.
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