[1] The daily averaged Solar EUV Monitor (SEM)/Solar Heliospheric Observatory (SOHO) EUV measurements, solar proxies, and foF2 data at 20 ionosonde stations in the east Asia/Australia sector are collected to investigate the solar activity dependences of the ionospheric peak electron density (NmF2). The intensities of solar EUV from the SEM/ SOHO measurements from 1996 to 2005 show a nonlinear relationship with F107, and the SEM/SOHO EUV can be better represented by a solar activity factor P = (F107 + F107A)/2. Seasonal and latitudinal dependences are found in the solar activity variation of NmF2 in the east Asia/Australian sector. The slope of NmF2 with P in the linear segment further shows similar annual variations as the background electron densities at moderate solar activity. Observations show a nonlinear dependence of NmF2 on solar EUV (the saturation effect of NmF2 for high solar EUV). On the basis of a simple model of photochemistry, taking the neutral atmospheric consequences into account, calculations at fixed height simulate the saturation effect of NmF2, but the observed change rate of NmF2 with P is inadequately reproduced. Calculations taking into account the influence of dynamics (via a simple model of the solar EUV dependence of the ionospheric height) tend to reproduce the observed change rate of NmF2. Results indicate that besides solar EUV changes, the influence of dynamics and the atmospheric consequences should substantially contribute to the solar activity variations of NmF2.

Circulating tumor cells (CTCs) are tumor cells that have sloughed off the primary tumor and extravasate into and circulate in the blood. Understanding of the metastatic cascade of CTCs has tremendous potential for the identification of targets against cancer metastasis. Detecting these very rare CTCs among the massive blood cells is challenging. However, emerging technologies for CTCs detection have profoundly contributed to deepening investigation into the biology of CTCs and have facilitated their clinical application. Current technologies for the detection of CTCs are summarized herein, together with their advantages and disadvantages. The detection of CTCs is usually dependent on molecular markers, with the epithelial cell adhesion molecule being the most widely used, although molecular markers vary between different types of cancer. Properties associated with epithelial-to-mesenchymal transition and stemness have been identified in CTCs, indicating their increased metastatic capacity. Only a small proportion of CTCs can survive and eventually initiate metastases, suggesting that an interaction and modulation between CTCs and the hostile blood microenvironment is essential for CTC metastasis. Single-cell sequencing of CTCs has been extensively investigated, and has enabled researchers to reveal the genome and transcriptome of CTCs. Herein, we also review the clinical applications of CTCs, especially for monitoring response to cancer treatment and in evaluating prognosis. Hence, CTCs have and will continue to contribute to providing significant insights into metastatic processes and will open new avenues for useful clinical applications.

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Purpose: Lignans such as secoisolariciresinol diglucoside in flaxseed, are metabolizes to bioactive mammalian lignans of END and ENL. Because mammalian lignans have chemical structural similarity to the natural estrogen, they are thought to behave like selective estrogen receptor modulators and therefore have anticancer effect against hormone-related cancers. We isolated a series of lignan compounds, named as Vitexins, from the seed of Chinese herb Vitex Negundo. Experimental Design: We purified several Vitexin lignan compounds. Cytotoxic and antitumor effects were analyzed in cancer cells and in tumor xenograft models. In vivo metabolism of Vitexins was determined in rat. Results: Contrasts to the classic lignans, Vitexins were not metabolized to END and ENL. A mixture of Vitexins EVn-50 and purified Vitexin compound 6-hydroxy-4-(4-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxy-3, 4-dihydro-2-naphthaldehyde have cytotoxic effect on breast, prostate, and ovarian cancer cells and induces apoptosis with cleavage in poly ADP ribose polymerase protein, up-regulation of Bax, and down-regulation of Bcl-2. This induction of apoptosis seems to be mediated by activation of caspases because inhibition of caspases activity significantly reduced induced apoptosis. We showed a broad antitumor activity of EVn-50 on seven tumor xenograft models including breast, prostate, liver, and cervical cancers. Consistent with in vitro data, EVn-50 treatment induced apoptosis, down-regulated of Bcl-2, and up-regulated Bax in tumor xenografts. Conclusion: Vitexin is a class of nature lignan compounds, whose action and anticancer effect is mediated by the mechanisms different from the classic lignans. Vitexininduced antitumor effect and cytotoxic activity is exerted through proapoptotic process, which is mediated by a decreased Bcl-2/Bax ratio and activation of caspases. (Clin Cancer Res 2009;15(16):5161-9)

Solar radiation, which varies over multiple temporal scales, modulates remarkably the evolution of the ionosphere. The solar activity dependence of the ionosphere is a key and fundamental issue in ionospheric physics, providing information essential to understanding the variations in the ionosphere and its processes. Selected recent studies on solar activity effects of the ionosphere are briefly reviewed in this report. This report focuses on (1) observations of solar irradiance at X-ray and extreme ultraviolet wavelengths and the outstanding problems of solar proxies, in the view of ionospheric studies, (2) new findings and improved representations of the features of the solar activity dependence of ionospheric key parameters and the corresponding physical processes, (3) possible phenomena in the ionosphere under extremely high and low solar activity conditions that are unique, as indicated by historical solar datasets and the deep solar minimum of solar cycle 23/24, and (4) statistical studies and model simulations of the ionosphere response to solar flares. The above-mentioned studies provide new clues for comprehensively explaining basic processes in the ionosphere and improving the prediction capability of ionospheric models and related applications.

[1] The electron density in the ionospheric F region occasionally stops its decay and rises pronouncedly during night hours, which are termed ionospheric nighttime enhancements. In this case study, we analyzed the manually scaled ionogram records measured by a Lowell DPS-4D ionosonde operated at Sanya (18.3°N, 109.6°E), China, to explore postmidnight enhancement events occurred in 2012, a year of moderate solar activities. Common features in these cases illustrate that, accompanying nighttime rises in peak electron density of F2 layer (NmF2), the height of F2 layer is depressed significantly, and the ionogram-derived electron density height profiles become thinner. There are time shifts in the development of electron density enhancements in the F layer; that is, enhancement develops earlier and reaches peaks earlier at higher altitudes than at lower altitudes. Meanwhile, plasma drift is detected downward under such events, revealing the essential role of the westward electric field in forming the postmidnight enhancements in electron density of ionospheric F layer at such low latitudes.

This paper shows that the relationship between solar EUV flux and the F10.7 index during the extended solar minimum (Smin) of 2007–2009 is different from that in the previous Smin. This difference is also seen in the relationship between foF2 and F10.7. We collected SOHO/SEM EUV observations and the F10.7 index, through June 2010, to investigate solar irradiance in the recent Smin. We find that, owing to F10.7 and solar EUV flux decreased from the last Smin to the recent one with different amplitudes (larger in EUV flux), EUV flux is significantly lower in the recent Smin than in the last one for the same F10.7. Namely, F10.7 does not describe solar EUV irradiance in the recent Smin as it did in the last Smin. That caused remarkable responses in ionospheric foF2. For the same F10.7, foF2 in the recent Smin is lower than that in the last one; further, it is also lower than that in other previous Smins. Therefore, F10.7 is not an ideal indicator of foF2 during the recent Smin, which implies that F10.7 is not an ideal proxy for solar EUV irradiance during this period, although it has been adequate during previous Smins. Solar irradiance models and ionospheric models will need to take this into account for solar cycle investigations.

A critical question in ionospheric physics is the state of the ionosphere and relevant processes under extreme solar activities. The solar activity during 2007–2009 is extremely prolonged low, which offers us a unique opportunity to explore this issue. In this study, we collected the global ionosonde measurements of the F2 layer critical frequency (foF2), E layer critical frequency (foE), and F layer virtual height (h′F) and the total electron content (TEC) maps produced by the Jet Propulsion Laboratory, which were retrieved from dual‐frequency GPS receivers distributed worldwide, to investigate the ionospheric phenomena during solar minimum of cycle 23/24, particularly the difference in the ionosphere between solar minima of cycle 23/24 and the preceding cycles. The analysis indicates that the moving 1 year mean foF2 at most ionosonde stations and the global average TEC went to the lowest during cycle 23/24 minimum. The solar cycle differences in foF2 minima display local time dependence, being more negative during the daytime than at night. Furthermore, the cycle difference in daytime foF2 minima is about −0.5 MHz and even reaches to around −1.2 MHz. In contrast, a complex picture presents in global h′F and foE. Evident reduction exists prevailingly in the moving 1 year mean h′F at most stations, while no huge differences are detected at several stations. A compelling feature is the increase in foE at some stations, which requires independent data for further validation. Quantitative analysis indicates that record low foF2 and low TEC can be explained principally in terms of the decline in solar extreme ultraviolet irradiance recorded by SOHO/SEM, which suggests low solar EUV being the prevailing contributor to the unusual low electron density in the ionosphere during cycle 23/24 minimum. It also verifies that a quadratic fitting still reasonably captures the solar variability of foF2 and global average TEC at such low solar activity levels.