Ocean acidification will potentially inhibit calcification by marine organisms; however, the response of the most prolific ocean calcifiers, coccolithophores, to this perturbation remains under characterized. Here we report novel chemical constraints on the response of the widespread coccolithophore species Ochrosphaera neapolitana (O. neapolitana) to changing-CO2 conditions. We cultured this algae under three pCO2-controlled seawater pH conditions (8.05, 8.22, and 8.33). Boron isotopes within the algae’s extracellular calcite plates show that this species maintains a constant pH at the calcification site, regardless of CO2-induced changes in pH of the surrounding seawater. Carbon and oxygen isotopes in the algae’s calcite plates and carbon isotopes in the algae’s organic matter suggest that O. neapolitana utilize carbon from a single internal dissolved inorganic carbon (DIC) pool for both calcification and photosynthesis, and that a greater proportion of dissolved CO2 relative to HCO3− enters the internal DIC pool under acidified conditions. These two observations may explain how O. neapolitana continues calcifying and photosynthesizing at a constant rate under different atmospheric-pCO2 conditions.
The response of marine-calcifying organisms to ocean acidification (OA) is highly variable, although the mechanisms behind this variability are not well understood. Here, we use the boron isotopic composition (δ11B) of biogenic calcium carbonate to investigate the extent to which organisms’ ability to regulate pH at their site of calcification (pHCF) determines their calcification responses to OA. We report comparative δ11B analyses of 10 species with divergent calcification responses (positive, parabolic, threshold, and negative) to OA. Although the pHCF is closely coupled to calcification responses only in 3 of the 10 species, all 10 species elevate pHCF above pHsw under elevated pCO2. This result suggests that these species may expend additional energy regulating pHCF under future OA. This strategy of elevating pHCF above pHsw appears to be a polyphyletic, if not universal, response to OA among marine calcifiers—although not always the principal factor governing a species’ response to OA.
The developed method for preparing and measuring boron isotopic values in a variety of carbonate materials should facilitate the reconstruction of past ocean pH conditions with decadal-scale resolution.
Abstract. The boron isotope composition (δ 11 B) of marine biogenic carbonates has been predominantly studied as a proxy for monitoring past changes in seawater pH and carbonate chemistry. However, a number of assumptions regarding chemical kinetics and thermodynamic isotope exchange reactions are required to derive seawater pH from δ 11 B biogenic carbonates. It is also probable that δ 11 B of biogenic carbonate reflects seawater pH at the organism's site of calcification, which may or may not reflect seawater pH. Here, we report the development of methodology for measuring the δ 11 B of biogenic carbonate samples at the multicollector inductively coupled mass spectrometry facility at Ifremer (Plouzané, France) and the evaluation of δ 11 B CaCO 3 in a diverse range of marine calcifying organisms reared for 60 days in isothermal seawater (25 • C) equilibrated with an atmospheric pCO 2 of ca. 409 µatm. Average δ 11 B CaCO 3 composition for all species evaluated in this study range from 16.27 to 35.09 ‰, including, in decreasing order, coralline red alga Neogoniolithion sp. (35.89 ± 3.71 ‰), temperate coral Oculina arbuscula (24.12 ± 0.19 ‰), serpulid worm Hydroides crucigera (19.26 ± 0.16 ‰), tropical urchin Eucidaris tribuloides (18.71 ± 0.26 ‰), temperate urchin Arbacia punctulata (16.28 ± 0.86 ‰), and temperate oyster Crassostrea virginica (16.03 ‰). These results are discussed in the context of each species' proposed mechanism of biocalcification and other factors that could influence skeletal and shell δ 11 B, including calcifying site pH, the proposed direct incorporation of isotopically enriched boric acid (instead of borate) into biogenic calcium carbonate, and differences in shell/skeleton polymorph mineralogy. We conclude that the large inter-species variability in δ 11 B CaCO 3 (ca. 20 ‰) and significant discrepancies between measured δ 11 B CaCO 3 and δ 11 B CaCO 3 expected from established relationships between abiogenic δ 11 B CaCO 3 and seawater pH arise primarily from fundamental differences in calcifying site pH amongst the different species. These results highlight the potential utility of δ 11 B as a proxy of calcifying site pH for a wide range of calcifying taxa and underscore the importance of using species-specific seawater-pH-δ 11 B CaCO 3 calibrations when reconstructing seawater pH from δ 11 B of biogenic carbonates.
Abstract. Ocean acidification, the decrease in ocean pH associated with increasing atmospheric CO2, is likely to impact marine organisms, particularly those that produce carbonate skeletons or shells. Therefore it is important to investigate how environmental factors (seawater pH, temperature and salinity) influence the chemical compositions in biogenic carbonates. In this study we report the first high-resolution strontium (87Sr / 86Sr and δ88 / 86Sr) and boron (δ11B) isotopic values in the aragonite shell of cultured Arctica islandica (A. islandica). The 87Sr / 86Sr ratios from both tank water and shell samples show ratios nearly identical to the open ocean, which suggests that the shell material reflects ambient ocean chemistry without terrestrial influence. The 84Sr–87Sr double spike resolved shell δ 88 / 86Sr and Sr concentration data show no resolvable change throughout the culture period and reflect no theoretical kinetic mass fractionation throughout the experiment despite a temperature change of more than 15 °C. The δ11B records from the experiment show at least a 5‰ increase through the culture season (January 2010–August 2010), with low values from beginning to week 19 and higher values hereafter. The larger range in δ11B in this experiment compared to predictions based on other carbonate organisms (2–3‰) suggests that a species-specific fractionation factor may be required. A relatively strong correlation between the Δ pH (pHshell-pHsw) and seawater pH (pHsw) was observed (R2 = 0.34), which suggests that A. islandica partly regulates the pH of the extrapallial fluid. However, this proposed mechanism only explains approximately 34% of the variance in the δ11B data. Instead, a rapid rise in δ11B after week 19 suggests that the boron uptake of the shell changes when a temperature threshold of 13 °C is reached.
Abstract. Ocean acidification, the decrease in ocean pH associated with increasing atmospheric CO 2 , is likely to impact marine organisms, particularly those that produce carbonate skeletons or shells. Therefore, it is important to investigate how environmental factors (seawater pH, temperature and salinity) influence the chemical compositions in biogenic carbonates. In this study we report the first highresolution strontium ( 87 Sr / 86 Sr and δ 88 / 86 Sr) and boron (δ 11 B) isotopic values in the aragonite shell of cultured Arctica islandica (A. islandica). The 87 Sr / 86 Sr ratios from both tank water and shell samples show ratios nearly identical to the open ocean, which suggests that the shell material reflects ambient ocean chemistry without terrestrial influence. The 84 Sr-87 Sr double-spike-resolved shell δ 88 / 86 Sr and Sr concentration data show no resolvable change throughout the culture period and reflect no theoretical kinetic mass fractionation throughout the experiment despite a temperature change of more than 15 • C. The δ 11 B records from the experiment show at least a 5 ‰ increase through the 29-week culture season (January 2010-August 2010), with low values from the beginning to week 19 and higher values thereafter. The larger range in δ 11 B in this experiment compared to predictions based on other carbonate organisms (2-3 ‰) suggests that a species-specific fractionation factor may be required. A significant correlation between the pH (pH shell − pH sw ) and seawater pH (pH sw ) was observed (R 2 = 0.35), where the pH shell is the calcification pH of the shell calculated from boron isotopic composition. This negative correlation suggests that A. islandica partly regulates the pH of the extrapallial fluid. However, this proposed mechanism only explains approximately 35 % of the variance in the δ 11 B data. Instead, a rapid rise in δ 11 B of the shell material after week 19, during the summer, suggests that the boron uptake changes when a thermal threshold of > 13 • C is reached.
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