Sulfated cyclofructan 6 (S-CF6) and sulfated cyclodextrins (S-α-, β-, γ-CDs) are highly selective chiral selectors for the enantioseparation of basic solutes. In this study, S-CF6 was introduced for the enantiomeric separation of four basic pharmaceuticals (including tamsulosin, tiropramide, bupivacaine, and norephedrine) by capillary electrophoresis (CE), and the enantiomeric separation performance was compared with S-α-, β-, γ-CDs. The effects of the chiral selector type, chiral selector concentration, operating voltage, and column temperature were examined and optimized. Excellent resolutions were obtained for all solutes on these chiral selectors.
A method of quickly determining tamsulosin enantiomers by capillary zone electrophoresis with a photodiode array detector was developed. Response surface methodologies based on three-level, three-variable designs such as Box-Behnken design, central composite face-centered, and central composite circumscribed design, were comparatively used for the optimization with respect to selector concentration, applied voltage, and column temperature. Statistical interpretation of the variables on different responses, such as resolution and migration time of the last isomer were performed. The optimum conditions of these variables were predicted by using a second-order polynomial model, fitted to the results obtained by applying three designs. The response surface plots using three experimental designs revealed a separation optimum with 100 mM tris (hydroxymethyl) aminomethane buffered with phosphoric acid to pH ¼ 2.5, concentration of sulfated-b-cyclodextrin, 0.15% (W/V), column temperature 258C, and applied voltage of 25 kV. The significance of the statistical designs was confirmed by the generally good agreement obtained between predicted responses and actual experimental data. We have concluded that experimental designs offer a rapid means of optimizing several variables, and provide an efficient test for the robustness of the analytical method.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.