Background: Msx1 and Msx2, which belong to the highly conserved Nk family of homeobox genes, display overlapping expression patterns and redundant functions in multiple tissues and organs during vertebrate development. Msx1 and Msx2 have well-documented roles in mediating epithelial-mesenchymal interactions during organogenesis. Given that both Msx1 and Msx2 are crucial downstream effectors of Bmp signaling, we investigated whether Msx1 and Msx2 are required for the Bmp-induced endothelial-mesenchymal transformation (EMT) during atrioventricular (AV) valve formation.
The o- and p-amino green-fluorescence-protein synthetic chromophores (GFPSCs) were synthesized from the corresponding o- and p-nitro protecting group. Among the four protecting groups of the o-amino group, the o-nitro protecting group is the only choice to synthesize the o-amino GFPSCs. The first singlet excited states of o- and p-amino GFPSCs carry significant charge-transfer character through the mechanism of photoinduced charge transfer (PCT). The o-amino GFPSCs can serve as wavelength-ratiometric fluorescence sensors that selectively recognize Cr(3+) in aqueous medium through a PCT mechanism.
Steep topographic reliefs and heavy vegetation severely limit visibility when examining geological structures and surface deformations in the field or when detecting these features with traditional approaches, such as aerial photography and satellite imagery. However, a light detection and ranging (LiDAR)-derived digital elevation model (DEM), which is directly related to the bare ground surface, is successfully employed to map topographic signatures with an appropriate scale and accuracy and facilitates measurements of fine topographic features. This study demonstrates the efficient use of 1-m-resolution LiDAR for tectonic geomorphology in forested areas and to identify a fault, a deep-seated landslide, and the regional cleavage attitude in southern Taiwan. Integrated approaches that use grayscale slope images, openness with a tint color slope visualization, the three-dimensional (3D) perspective of a red relief image map, and a field investigation are employed to identify the aforementioned features. In this study, the previously inferred Meilongshan Fault is confirmed as a NE-SW-trending, eastern dipping thrust with at least a 750 m-wide deformation zone. The site where future paleoseismological studies should be performed has been identified, and someone needs to work further on this site. Signatures of deep-seated landslides, such as double ridges, trenches, main escarpments, and extension cracks, are successfully differentiated in LiDAR DEM images through the use of different
The excited state intramolecular proton transfer (ESIPT) that occurs in the o-sulfonamide analogue ( o-TsABDI) of the green fluorescent protein (GFP) chromophore provides an alternative model to get insights into the excited state proton transfer (ESPT) related photophysics of GFP. In this article, we explored the ESIPT-related photophysics of o-TsABDI by electronic absorption and fluorescence emission spectra in a wide polarity range of solvents, cis- trans photoisomerization experiment, and Coulomb-attenuating method (CAM)/time-dependent (TD) density functional theory (DFT) calculations. We found that the whole ESIPT process involves four steps. The first step is photoexcitation of o-TsABDI, which does not involve charge transfer (CT). The second step is ESIPT and accompanying electron transfer from the n orbital of the sulfonamide nitrogen to the half-filled π orbital of the 4-benzylideneimidazolone moiety. The third step is fluorescence emission of the zwitterionic o-TsABDI and accompanying CT from the π* orbital of the 4-benzylideneimidazolone moiety to the half-filled n orbital of the sulfonamide nitrogen. The last step involves irreversible and barrierless proton recombination. In contrast to the isolated GFP chromophore and its p- and m-amino analogues, the S excited state of o-TsABDI does not relax by way of cis- trans photoisomerization through the S/S conical intersection CI(I) by rotating around the I-bond, but follows the ESIPT pathway. The low fluorescence quantum yield of the zwitterionic o-TsABDI might be due to (1) the fluorescence that involves the low-probability π* → n charge transfer and (2) nonradiative relaxation through the S/S conical intersection CI(P″) by rotating around the P-bond.
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