Aims: This study was designed to compare the extractable yield of Chrysophyllum albidum seed oil, the phytoconstituents, and physicochemical parameters with the commercially available vegetable oil, to ascertain their suitability for human consumption and industrial uses. Place and Duration of Study: Biomedicinal Research Centre, Forestry Research Institute of Nigeria in collaboration with the Pharmaceutical Chemistry Laboratory, University of Ibadan, Oyo State, Nigeria, between October, 2018 to July, 2019. Methodology: The seeds were collected from two locations (a parent tree in the Forestry Research Institute of Nigeria (FRIN) and as well procured at Akesan market in Oyo town (Oyo) all in Oyo State, Nigeria. The experiments were executed adopting the standard procedures. The air-dried powdered C. albidum seed was cold macerated with analytical grade N-Hexane. The oils were purified using activated charcoal and qualitatively screened to ascertain the phytochemicals in them. Physico-chemical parameters were quantitatively determined following AOAC guidelines. Results: The results revealed that C. albidum is a low oil yielding seed especially when cold maceration was employed. The phytochemical screening revealed the presence of alkaloids, anthraquinones, terpenoids, and cardiac glycosides in all the oils. Saponins were found only in the oil from the FRIN source. While tannins and flavonoids were absent in all the oils. The physico-chemical parameters revealed the ranges of 0.90 - 9.45 mgKOH/g (Acid value), 101.90 - 356.60 mgKOH/g (saponification value), 65.30 - 78.00 mg/g (iodine value), 101.00 - 348.50 (ester value), 2.93 - 6.21 (PH value), 0.787 - 0.900g/cm3 (Relative density) and 1.4590 - 1.6560 (Refractive index @280C). Conclusion: It can be deduced that there are disparities in the yield, phytoconstituents and the physico-chemicals of the oils used for this study. Further research is needed on the C. albidum oil to validate its edibility and affirm its medicinal uses.
A B S T R A C TTomato is a commercially important vegetable throughout the whole world and its availability all the year is grossly affected by anthracnose disease, hence, the need for an effective bio-control that is affordable and user friendly. This study therefore investigated the inhibitory effect of ethanol extracts of Azadirachta indica and Tabacum nicotianaon the mycelium growth of fungi associated with anthracnose disease of tomato. Tomatoes that showed black circular lesions with concentric ring and black spores were bought from Aleshinloye market in Ibadan; samples (3×3mm) from the sterilized margin of the lesion were inoculated on potato dextrose agar and observed for mycelium growth for five days. The obtained cultures were then sub cultured to obtain pure cultures which were introduced into healthy tomato fruits to confirm koch's postulate. The in-vitro antifungal effects of ethanol extracts of Azadirachta indica and Nicotiana tabacum on isolated fungus causing anthracnose disease of tomato was investigated using agar dilution method. Based on cultural and microscopic characteristics of conidia, Colletotrichum coccodewas identified as organism causing anthracnose disease of tomato. The antifungal effect of the two ethanol plant extracts revealed that Azadirachta indica had the highest inhibitory effect of (45.00cm) at 100mg/ml and the least effect of (38.00cm) at 50mg/ml. Nicotiana tobacum equally had its highest inhibitory effect of (58.00cm) at 100mg/ml and lowest effect of (40.57cm) at 50mg/ml. This result also showed that Nicotiana tobacum had the highest inhibitory effect on Colletotrichum coccode than Azadirachta indica. Conclusively, the use of Tobacco and Neem plant extracts has antifungal activity against Colletotrichum which is responsible for Anthracnose disease of tomato. The plant could be a veritable and cheaper substitute for conventional drugs since the plant is easily obtainable and the extract can easily be made via a simple process of maceration or infusion.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.