Hydrodistillation of cajuput (Melaleuca cajuputi) leaves collected from 6 sites in Narathiwat gave different yields of cajuput oils. The maximum oil yield (0.97%) was obtained from leaves from Ban Koke Kuwae, Thambon Kosit, and Amphur Tak Bai. The oil yields from leaf samples of other sites were 0.84% from Ban Pha Ye and Thambon Sungai Padi in Amphur Sungai Padi; 0.76% from Ban Lubosama, and Thambon Pasemat, in Amphur Sungai Kolok; 0.70% from Ban Tha Se, and Thambon Kosit, in Amphur Tak Bai; 0.66% from Ban Mai, and Thambon Sungai Padi, in Amphur Sungai Padi; and 0.56% from Ban Toh Daeng, and Thambon Phuyoh, in Amphur Sungai Kolok. Cajuput oil densities from the 2 sites of Amphur Sungai Kolok and from Ban Mai, Thambon Sungai Padi, Amphur Sungai Padi were almost the same, but higher than others. Although major components were not different, the minor components varied in terms of both structure and proportion. The major compositions of both cajuput oils from Ban Toh Daeng, Thambon Phuyoh, and Amphur Sungai Kolok consisted of 49.22% monoterpenes and 46.45% sesquiterpenes, and the rest were hydrocarbons and a diterpene. Other cajuput oils obtained composed mainly of monoterpenes (more than 62%), sesquiterpenes, hydrocarbons and some unknown compounds respectively. There was no diterpene present in these oils. Since cajuput oil was locally used as insecticide, termicidal activities of all oils were also investigated.
Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy (37°C, 6 weeks) and chemotherapy(Ribavirin Ⓡ). The detection of apple viruses was accomplished by Enzyme-linked Immuno-Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR method was 10~30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30~40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.
Plant growth in a controlled environment system is highly dependent on the availability of light. The light-emitting diode (LED) is capable of providing the needed quality and quantity of light for the plant. The purpose of this study was to determine the effect of white LED light intensity on the growth of in vitro propagated apple (M-9) seedlings in a controlled environment system. Seedlings were grown for 30 days under five different white LED light intensities: 100–500 (L1), 250–500 (L2), 500–500 (L3), 250–250 (L4), and 100–100 (L5). Our findings indicate that seedlings treated with L3 grew substantially taller than seedlings treated with L1, L2, or L5. The number of leaves, stem diameter, shoot fresh weight, root fresh weight, and shoot dry weight of L3 treated seedlings were considerably greater than those growing in other treatments. Furthermore, root length, root dry weight, chlorophyll content, and photosynthesis rate were considerably increased in the L3 treatment group compared to the L5 treatment group. However, there was no significant difference in the stomatal conductance or transpiration rate of apple seedlings between the light treatments. Moreover, a positive correlation was seen between stomatal conductance and transpiration rate. These results suggest that light intensity PPFD 500-500 were favorable for the initial growth of in vitro propagated apple seedlings.
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