Primary leaf explants of aseptically grown seedlings of moth bean [Vigna aconitifolia (Jacq.) Marechal] immersed in water or not were treated in microwave oven (2450 MHz, 800 W cm -2 ) for 1, 3, 5 and 7 s before culturing. Callusing and shoot emergence from these explants were enhanced up to microwave exposure lasted 5 s while longer treatment of water-immersed explants delayed callusing. One polypeptide (26.6 kD) was up regulated in the callus derived from microwave treatment in water-immersed explants. RAPD analysis detected alteration in DNA sequences due to microwave treatment in water-immersed explants for 7 s. The frequency of mutation was 1.6 % (4 bands out of 248) over all the cultures analyzed and the same was 13 % (4 bands out of 31), if amplicons generated at 7 s treatment alone were considered.Additional key words: in vitro culture, moth bean, RAPD.
In this work, microbial community structure of two distinct arid plants like ker (Capparis deciduas) and pearl millet (Pennisetum glaucum) was assessed and defined by culture-dependent and cultureindependent approaches on the basis of 16S rRNA and random amplified polymorphic DNA (RAPD) analysis. The average Jaccard's similarity coefficient values for cultivated bacteria that is within ker and pearl millet rhizosphere were 0.701 and 0.707, respectively, for non-rhizosphere of ker and pearl millet 0.739 and 0.762, respectively, and for non-cultivable bacteria under ker (0.519) and under pearl millet (0.534). Both culture-dependent and culture-independent methods indicated that in arid crops, microbial diversity is more influenced by soil type rather than plant type and lower Jaccard value for metagenome showed that whole community harbours more diversity because of different microflora than cultivated only. Salinity and temperature tolerance study of bacteria indicated that ker rhizosphere harbours more salinity and temperature tolerant bacteria.
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