Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial transport using microfluidic-chamber-cultured neurons together with a newly developed analysis package named “MitoQuant”. This tool-kit consists of an automated program for tracking mitochondrial movement inside live neuronal axons and a transient-velocity analysis program for analyzing dynamic movement patterns of mitochondria. Using this method, we examined axonal mitochondrial movement both in cultured mammalian neurons and in motor neuron axons of Drosophila in vivo. In 3 different paradigms (temperature changes, drug treatment and genetic manipulation) that affect mitochondria, we have shown that this new method is highly efficient and sensitive for detecting changes in mitochondrial movement. The method significantly enhanced our ability to quantitatively analyze axonal mitochondrial movement and allowed us to detect dynamic changes in axonal mitochondrial transport that were not detected by traditional kymographic analyses.Electronic supplementary materialThe online version of this article (doi:10.1007/s13238-016-0268-3) contains supplementary material, which is available to authorized users.

Conventional deconvolution methods assume that the microscopy system is spatially invariant, introducing considerable errors. We developed a method to more precisely estimate space-variant point-spread functions from sparse measurements. To this end, a space-variant version of deblurring algorithm was developed and combined with a total-variation regularization. Validation with both simulation and real data showed that our PSF model is more accurate than the piecewise-invariant model and the blending model. Comparing with the orthogonal basis decomposition based PSF model, our proposed model also performed with a considerable improvement. We also evaluated the proposed deblurring algorithm. Our new deblurring algorithm showed a significantly better signal-to-noise ratio and higher image quality than those of the conventional space-invariant algorithm.

The location of the pathological focus on the film of CT scan was related to the degree of paralysis, and to the result of acupuncture in hemiplegia patients. In retrospective analysis of the clinical data, we found some inter-linking relationships. In general, early treatment (first 3 weeks) with acupuncture produces better result (improvement in 90.9% of the patients treated) than the treatment initiated three weeks after stroke (improvement in 71.4% of the patients treated).

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Combining acupuncture with acupoint-injection of aceglutamidi has been used in treating 38 cases of senile dementia. Our experiment showed that the therapy is effective for the cases of multi-infarct dementia, the rate of success being 42.85% and of improvement 42.86%, the total efficacy rate being 85.71%. The rating was based on the revised Hasegawa Dementia Scale and the Functional Activity Questionnaire. In addition, it has been observed that the component of high density lipid-cholesterone increased significantly after treatment.

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Découverte archéologique 1973 Changsha (Hunan, Chine) SILK SCROLLS :earliest literature of meridian doctrine in ancient china. Yemeng Chen. acupuncture and electrotherapeutics research, 1997, 22(3-4), 175antérieur au nei jing su wen et ling shu compilés au 2ème siècle av. JC (1er siècle après JC) antérieur au nei jing su wen et ling shu compilés au 2ème siècle av. JC (1er siècle après JC) 20 livres sur rouleaux de soie ou sur bambou 20 livres sur rouleaux de soie ou sur bambou Mawangdui tombe n°3 Li Cang "marquis de Tai" 186 av.. JC Mawangdui tombe n°3 Li Cang "marquis de Tai" 186 av.. JC canon de la moxibustion des onze méridiens des jambes et des bras canon de la moxibustion des onze méridiens yin et yang (deux versions légèrement différentes d'un même texte) compilés au 3ème siècle av. JC canon de la moxibustion des onze méridiens des jambes et des bras canon de la moxibustion des onze méridiens yin et yang (deux versions légèrement différentes d'un même texte) compilés au 3ème siècle av. JC le contenu des manuscrits sur soie de Mawangdui

In the current crisis of abuse and misuse of prescription opioids, acupuncture is considered as an important non-pharmacological pain management modality. Acupuncture not only takes care of pain symptoms but also deals with whole body balance. It is a drug-free, cost-effective, and harmless therapy with a tremendous number of indications. Scientific data shows favorable outcomes towards pain management and also for drug addiction. Advanced acupuncture therapies have provided more opportunities for pain management. The mainstream medical field has accepted acupuncture in a favorable way, but the profession should also enhance their knowledge and competencies in order to provide a higher standard of acupuncture treatment.