BackgroundPolysaccharides from bivalves have multiple bioactivities in various aspects of biology. However, the role of a polysaccharide derived from Amusium pleuronectes on potential hepatoprotective effects remains unclear.Material/MethodsA water-soluble polysaccharide was isolated from Amusium pleuronectes (APS-1) using ultrasound-assisted hot-water extraction. The molecular weight of APS-1 was approximately 11.7 kDa and was determined by calibration with dextran. APS-1 was analyzed by high-performance liquid chromatography (HPLC), and mainly consisted of a uniform glucose polymer. The protective effect of APS-1 on Schistosoma japonicum-induced liver fibrosis was investigated in a mouse model.ResultsTreatment with APS-1 increased serum levels of interleukin (IL)-12 and interferon (IFN)-γ, increased superoxide dismutase (SOD) activity, and decreased levels of IL-13 and IL-5, and hyaluronidase activity. Moreover, immunohistochemical analysis revealed that the collagen content of hepatic tissue of APS-1-treated mice, including that of collagen I, II, and IV, was dramatically decreased. Furthermore, our data showed that combined treatment of APS-1 with praziquantel had more pronounced effects than treatment with either APS-1 or praziquantel alone.ConclusionsOur findings suggest that the treatment using APS-1 in combination with praziquantel attenuated S. japonicum egg-induced hepatic fibrosis, and possessed potent hepatoprotective activity.
As a key regulatory molecule in neurological disorders, the mechanism by which Rab10 exerts its protective effect in neuronal cells in depression is currently unknown. This research aimed to explore the function and mechanism of action of Rab10, a gene associated with neuroprotection, by using an in vitro model of depression. PC12 cells induced by corticosterone (CORT) were used to simulate depression in vitro. The viability of PC12 cells was detected using a CCK-8 assay, and the interaction between miRNA-103-3p and Rab10 was confirmed by bioinformatics combined with double luciferase and RNA Binding Protein Immunoprecipitation (RIP) experiments. The level of miRNA-103-3p and Rab10 were detected using a quantitative PCR assay. The protein contents of Rab10, BDNF, CREB, P62, Beclin-1, Wnt3a, GSK3β, phosphorylated (p)-GSK3β, and β-catenin were detected by western blotting. The results indicated that the content of Rab10 was downregulated in CUMS rats and CORT-induced PC12 cells. Bioinformatics combined with double luciferase and RIP experiments showed that miRNA-103-3p targeted Rab10. Overexpression of Rab10 or silencing of miRNA-103-3p in CORT-induced PC12 cells activated the Wnt/β-catenin signaling pathway, upregulated the contents of BDNF, CREB, and Beclin-1, but downregulated the expression of P62 protein, whereas silencing Rab10 based on silencing miRNA-103-3p reversed the effect of miRNA-103-3p. Overall, our data indicated that miRNA-103-3p targeted Rab10 to activate the Wnt/β-catenin signaling pathway to increase cellular nerve plasticity and promote autophagy, thus resisting CORT-induced damage to PC12 cells.
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