RESUMENObjetivos. Investigar los efectos del D-002, mezcla de seis alcoholes alifáticos primarios de alto peso molecular, obtenida de la cera de abejas (Apis mellifera), sobre la colitis ulcerativa (CU) inflamatoria severa inducida por sulfato de dextrano (DSS) y etanol en ratas (Ratus ratus). Materiales y métodos. Las ratas se distribuyeron aleatoriamente en seis grupos: un control cero al que no se provocó daño, y cinco a los que se les indujo la CU: un control negativo (vehículo), tres tratados con D-002 (25, 100 y 400 mg/kg) y un control positivo con sulfazalacina (200 mg/kg) (sustancia de referencia). Se cuantificaron las manifestaciones clínicas (variación del peso corporal, presencia de diarrea y de sangrado rectal), el puntaje de daño macroscópico e histológico, y la actividad de mieoloperoxidasa (MPO). Resultados. El tratamiento oral con D-002 (25, 100 y 400 mg/kg) previno significativamente la disminución del peso corporal. La dosis de 400 mg/kg redujo la presencia de diarreas y sangrado rectal, aunque su comparación con el control negativo solo alcanzó significación estadística sobre las diarreas. El D-002 (25, 100 y 400 mg/kg) redujo significativamente el puntaje de las lesiones macroscópicas (40,0; 43,3 y 47,2% de inhibición, respectivamente), el puntaje de daño histológico (31,5; 53,7 y 67,1% de inhibición, respectivamente) y la actividad de MPO (73,2; 83,6 y 85,0% de inhibición, respectivamente), comparado con el grupo control negativo. La sulfazalacina redujo significativamente todas las variables estudiadas. Conclusiones. El D-002 (25, 100 y 400 mg/kg) protegió significativamente la mucosa colónica en ratas con CU inflamatoria severa inducida por DSS y etanol. Palabras clave: Abejas; Colitis ulcerosa; Ratas (Fuente: DeCS BIREME). EFFECTS IN RATS OF BEE-WAX ALCOHOLS (D-002) ON ULCERATIVE COLITIS INDUCED BY DEXTRAN SULFATE AND ETHANOL ABSTRACTObjectives. To investigate the effects of D-002, a mixture of 6 high molecular weight primary aliphatic alcohols, obtained from beeswax (Apis mellifera), on severe inflammatory ulcerative colitis (UC) induced by Dextran sulfate (DSS) and ethanol in rats (Ratus ratus). Materials and methods. Rats were randomly distributed in six groups: a zero control to which no damage was caused, and five to which the UC was induced: a negative control (vehicle), three treated with D-002 (25, 100 and 400 mg/kg) and a positive control with sulfasalazine (200 mg/kg) (reference substance). Clinical manifestations (body weight variation, diarrhea and rectal bleeding), macroscopic and histological damage score, and myeloperoxidase (MPO) activity were quantified. Results. The oral treatment with D-002 (25, 100 and 400 mg/ kg) significantly prevented the decrease in body weight. The dose of 400 mg/kg reduced the presence of diarrhea and rectal bleeding, although its comparison with the negative control only reached statistical significance on diarrhea. D-002 (25, 100 and 400 mg/kg) significantly reduced the score of macroscopic lesions (40.0; 43.3 and 47.2% inhibition, respectively...
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