To clarify new bone tissues, radiography with a newly developed tunable wavelength and
highly parallel Parametric X-ray, PXR, was applied. Methods: PXR was generated by a LINAC at
LEBRA, Nihon University (Hayakawa et al., 2005). X-ray wavelength was tuned from 7 KeV, 0.177
nm, to 16 KeV, 0.0775 nm. Coated or grit-blasted Ti-alloy implants modified with coating or blasting
were implanted in surgically created defects in rabbit tibia. Undecalcified polished thin sections were
prepared from the implant/bone areas 1 and 4 weeks after implantation. Results: PXR radiography
showed a distinct difference between the newly formed bone and the compact bone. Color-mapping
of the images showed an increase in the total amount of bone formation with time. Conclusion:
Application of LEBRA-PXR, a high-powered, highly monochromatized and highly parallel oriented
X-ray allowed easy and accurate radiographic analysis of new bone formation around the implant.
We obtained interesting results when analyzed the bones of the aged rats by the Micro-XRD. The samples were in a rat thighbone after 96 weeks and in rat thighbone after 8 weeks and 48 weeks as control group. The samples were analyzed ten points and 1,800 sec by the Micro-XRD. The crystal after 96 weeks was worse than control group.
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