In this study 13 leech species from Tunisia are listed. They belong to 2 orders, 2 suborders, 4 families and 11 genera. The paper includes also data about hosts and habitats, distribution in the world and in Tunisia. Faunistic informations on leeches were found in literature and in the results of recent surveys conducted by the authors in the North East and the South of the country. The objectives of this study were to summarize historical and recent taxonomic data, and to propose an identification key for species signalized. This checklist is to be completed, taking into account the hydrobiological network of the country especially the North West region, which may reveal more species in the future

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