Vitiligo is a developed depigmentation disorder consisting of two forms which are segmental vitiligo (SV), affect one side of the body in 50% of individuals and non-segmental vitiligo (NSV) which is more common and affects both side of the body. A studydocumented that vitiligo susceptibility genes that are linked to immune regulation and immune targeting of melanocytes which are Tumor necrosis factor alpha (TNF-α), Interferon gamma (IFN-γ) and interleukin 10 (IL-10), are responsible for the pathogenesis of vitiligo. This study was designed to shed light on cytokinesand oxidative stress which may play a critical role in the pigmentary process of NSV.Fifty NSV patients were collected from dermatology department Baghdad teaching hospital, Baghdad, Iraq. And fifty healthy volunteers' individuals enrolled in this study during the period from November 2018 to May 2019. Some parameters including the concentration of immunological guideline TNF-α, IFN-γ and IL-10, and the level of oxidative stress H2O2 in malondialdehyde (MDA) form and superoxide dismutase SOD were evaluated in sera using ELISA technique.Serum level of TNF-α and IFN-γ were significantly higher in patient as compared to control. While serum IL-10 was significantly lower in vitiligo when compared with healthy.The assayed serum level of oxidative stress MDA and superoxide dismutase was found to be increased in vitiligo.There was an imbalance between pro and anti-inflammatory cytokines. Elevated levels of MDA and SOD suggested that an oxidative stress and antioxidants could play an adjuvant role in the management of vitiligo in addition to specific therapies.
The aim: This study was designed to investigate the potential role of miRNA-155 in the pathogenesis of HPV-induced cervical cancer.
Materials and methods: A total of 42 formalin-fixed paraffin-embedded (FFPE) cervical cancer tissue samples and 38 FFPE normal cervical tissue samples were used (they were collected at the Department of Pathology, Baghdad teaching hospital, Baghdad, Iraq, between January 2019 to January 2021). Following HPV testing and genotyping, the expression of miRNA-155 were evaluated by real-time PCR (qPCR).
Results: A statistically significant up-regulation of miRNA-155 expression was observed in cervical cancer tissues compared to results in control group, regardless of HPV status and clinical grading.
Conclusions: These data suggest that overexpression of miRNA-155 can delineate cervical cancer tissues from normal and may be a useful diagnostic biomarker for early detection of cervical cancer.
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