Amelogenin is a complex enamel matrix protein that consists of various molecular-size proteins and amino acids. A spliced form of amelogenin was identified that included exons 2, 3, 5, 6, and 7. However, the biological function of amelogenin exon 5 on dental pulp remains unknown. We designed a synthetic amelogenin exon 5 encoded peptide (SP), which was based on a protein produced by cells in response to the enamel matrix derivative (EMD). We investigated the effect of the SP on potentiation of osteogenesis and its signal pathway in dental pulp stem cells (DPSCs). DPSCs are an important cell for pulp tissue homeostasis. DPSCs were cultured with SP to examine the effect of cell proliferation and osteogenic differentiation. We also investigated the mitogen-activated protein kinase (MAPK) signaling pathway. SP significantly enhanced cell proliferation and the expression of osteogenic differentiation. Moreover, SP promoted the expression of the MAPK signaling pathway. Therefore, amelogenin exon 5 might contribute to dental pulp capping.
Enamel matrix derivative (EMD) is used for periodontal tissue regeneration therapy. We designed a synthetic amelogenin peptide (SP) derived from EMD, and have previously investigated the biological function of SP. However, it is unknown whether SP affects odontoblastic differentiation. In the present study, we investigated the effects of SP in odontoblast-like cells, KN-3 cells. KN-3 cells were treated with SP (0, 1, 10, 100, or 1000 ng/mL) and then cultured for 3, 8, 24, or 48 hours, in order to determine the effects of SP on cell proliferation and detect its optimum concentration. To investigate the effect of SP on odontogenic differentiation, KN-3 cells were treated with SP in odontogenic differentiation medium cultured for 3 or 7 days. Odontogenic differentiation was performed by measuring alkaline phosphatase (ALP) activity, the mRNA expression of dentin sialophosphoprotein (DSPP), the formation of calcified nodules, and calcium deposition into the extracellular matrix. The addition of SP significantly promoted KN-3 cell proliferation; a concentration of 100 ng/ml generated the greatest change in cell proliferation. SP also showed increased expression of markers of odontogenic differentiation and mineralization. These results suggest that SP, derived from EMD, could be a potential for applicate to the dental pulp capping.
Enamel matrix derivative (EMD) is applied for periodontal therapy. We created a synthetic amelogenin peptide (SP) derived from EMD, and have previously investigated the biological function of SP. However, it is unknown whether SP affects odontoblastic differentiation. In this study, we tested the effects of SP in the odontoblast-like cells, KN-3 cells. KN-3 cells were cultured with SP (0 to 1000 ng/mL) and then cultured for 3, 8, 24, or 48 h in order to determine the effects of SP on cell proliferation and detect its optimum concentration. KN-3 cells were treated with SP in odontogenic differentiation medium cultured for 3 or 7 days. Odontogenic markers were measured by the detection of alkaline phosphatase (ALP) activity and dentin sialo phosphoprotein (DSPP) expression, the calcified nodule formation, and calcium deposition. The addition of SP significantly promoted cell proliferation at 100 ng/mL, generating the greatest change in cell proliferation. SP also showed increased odontogenic expression markers and mineralization. These results suggest that SP, derived from EMD, could have potential for application in dental pulp capping.
Enamel matrix derivative (EMD) is used for periodontal tissue regeneration therapy, and
17can induce mineralization in dental pulp cells (DPCs). We designed a synthetic peptide (SP) derived
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